Rotifer in action

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NikonUser
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Rotifer in action

Post by NikonUser »

Finally received some Hydroxyethyl cellulose (Protoslo) from the US and tried it out on a very active, free swimming, rotifer.
On a microscope slide, added one drop to a drop of water containing the rotifer. This stopped the rotifer from swimming but did not stop its toes from moving (nuisance) and did not stop its feeding action.
It appears that the Protoslo did not completely mix with the water and at least one 13.6µ droplet existed.
In this stack of 27 frames this droplet (bottom of image) can be seen in its curved course to finally enter the rotifer’s mouth. Another current is apparent on the upper surface.
This is an artificial situation in that the rotifer was constrained between a microscope slide and a cover glass.
Olympus 20x S Plan Apo, NFK 2.5x projection eyepiece; 27 frames @ 2µ, flash, full frame; ZS P Max stack
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NUM10083
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Mitch640
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Post by Mitch640 »

Interesting catch. How long between shots?
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NikonUser
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Post by NikonUser »

Mitch:
Are you going to calculate the speed of the water flow?
Time between the 1st and last droplet 3min 20 sec.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

I was curious how fast it moved, but that's really slower than I thought. Usually, you see small objects go whizzing by at fractions of a second per body length.
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NikonUser
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Post by NikonUser »

Remember, this is not pure water but a syrupy thick soln of Protoslo.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
Top
Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Ah, I have not tried mine yet, so didn't know if it actually thickened the water drop or chemically "slowed" the living things.
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