Pollen
Moderators: rjlittlefield, ChrisR, Chris S., Pau
- rjlittlefield
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Pollen
Shot using Nikon M PLan 20X/0.40 ELWD objective in an Olympus CH microscope frame. Canon T1i in LiveView mode at ISO 100, 1/13 second exposure, illuminated with halogen fiber through a Kleenex tissue diffuser. Zerene Stacker DMap with retouching from original source frames using PMax for guidance. Finished in Photoshop with smart sharpen, levels adjustment, and some masking to avoid noise in the background.
304 frames at 1 micron, using a StackShot controller and stepper motor to drive the fine focus knob of the microscope. It's a simple arrangement. I'll show it over in Micro Technique later today.
Hope you enjoy!
--Rik
- rjlittlefield
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