Leitz Ortholux microscope
4X Leitz projection eyepiece plus 1/3x relay lens
Image No.1
257 images at 2.5 micron increments
Olympus 10x S Plan achromat objective
Leitz dry darkfield condenser D 0.80
Image No. 2,
87 images at 5 micron increments
Olympus 10x S Plan achromat objective
Reflected light, diffused fiber optic illumination
Live subject
Image No.3
69 images at .001 inch increments
Leitz 4x Fluorite Plan achromat objective
Leitz dry darkfield condenser D 0.80
Canon 50D
Zerene and Photoshop processing.
Image 2 is of a embryo visible to the far left in Spiders No. 12 while the egg ball was still intact and held up by the mother. Slight movements by the mother at this magnification limited the number of images, reducing image depth. It was decided to get the mother to release her brood.
In a struggle with the spider to release her egg ball, the bonds were damaged allowing some eggs to be released. They are extraordinarily fragile and sticky making handling very difficult. Image number 1 was made from what remained with the mother. All were frozen to allow for extensive stacking.
Walt
