Zygnema & Hyalotheca dissilens -2nd image added

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NikonUser
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Zygnema & Hyalotheca dissilens -2nd image added

Post by NikonUser »

EDIT: partial title changed, was "....& unknown algae...."
Hyalotheca is a colonial filamentous Desmid (thanks Jan)

From a coastal FW lake.

This unbranched filamentous algae has long cylindrical cells each containing
2 stellate chloroplasts and a nucleus in a cytoplasmic bridge between the two.

The thin mucilaginous sheath can be seen in the lower image. Compare the width of the Zygnema sheath with that of the unknown filamentous
algae (ID apreciated) in the lower image. OOF Spirogyra below.

Oly 40x S Plan Apo, NFK 2.5x photoeyepiece. ZS PMax stacks of 1µ frames.
Flash
Image
NUM10034
Last edited by NikonUser on Sat Sep 04, 2010 8:30 am, edited 2 times in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Mitch640
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Post by Mitch640 »

Every time I see one of your images, I learn something. I was looking at filament algae yesterday, and saw the sheath, but thought it was cilia that was moving too fast to see well. LOL

What camera are you using to capture your images? I don't believe I ever heard.
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NikonUser
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Post by NikonUser »

Thanks Mitch.
I have an Olympus BHS trinocular microscope with a NFK 2.5x photoeyepiece.
It's a very simple matter to support a DSLR camera with bellows (no lens) over the photoeyepiece (see Charles Krebs wed page for how-to).

I am using a Nikon D90 which has a HDMI port so that I can see the preview image in Live View and see each exposed frame on a HDMI TV.

2nd image of this unknown algae; underexposed to show the wide mucilage sheath and normal exposure to give an idea of cell contents.
Image
NUM10035
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Thanks for the clarification. I am still fighting adapter problems with both my Canon DSLR and my dedicated video camera for the scope. I'll eventually get it though. Your images are always so good, I had to ask. So far, direct viewing gives wonderful viewing, but I haven't been able to capture a really nice image yet. :)
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Jan Kros
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Post by Jan Kros »

Hello Nu,

The other algae is Hyalotheca dissilens
Greetings
Jan
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NikonUser
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Post by NikonUser »

Many thanks, Jan.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
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mkonikoff
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Location: WASHINGTON, USA

Post by mkonikoff »

Image

Hyalotheca collected at Derby Pond in Bellingham WA, on 12 August 2014
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carlos.uruguay
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Post by carlos.uruguay »

Interesting
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Olympusman
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Mucilage sheath

Post by Olympusman »

I think many of us have found trying to capture that mucilage sheath a very frustrating task. It looks great in darkfield, but I can't decent darkfield past a 10x objective. Considering this difficulty, your capture is pretty good.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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