Difflugia urceolata (attractive test)

Charles Krebs · Post by **Charles Krebs** » Thu Aug 05, 2010 11:47 pm

Amoeba tests never cease to amaze me! Microscopic pottery made by a single cell craftsman. This was a very nice Difflugia urceolata

Olympus BHS, 20/0.46 S Plan Achromat, 1.67 NFK photoeyepiece, brightfield illumination, Canon DSLR.

Jean-marc · Post by **Jean-marc** » Fri Aug 06, 2010 10:17 am

Beautiful Charles,

You always have the good idea to show us stunning object

JM

rjlittlefield · Post by **rjlittlefield** » Fri Aug 06, 2010 11:55 am

Very nice!

Looking at the right side near the opening, are those pseudopodia I see? Was this a live specimen?

--Rik

Charles Krebs · Post by **Charles Krebs** » Fri Aug 06, 2010 2:17 pm

Looking at the right side near the opening, are those pseudopodia I see? Was this a live specimen?

Yes. It is a 39 image ZS stack. There were many pseudopodia emerging and moving about, so after running the stack I retouched using a single frame where they showed a little. With brightfield illumination, and the test being so much darker than the clear pseudopodia, they barely registered (overexposed) at the proper exposure for the "shell" (actually they don't show up well in "straight" brightfield in any event).

BTW... this is just sitting on top of the slide in a drop of water. I did not want to use a cover slip since it is too easy to accidentally break these with a cover slip. That's why I used the 20/0.46 Achromat... the higher NA 20X I have does not do well at all with no coverglass (and I don't use the M Plans on this BHS microscope).