Spiders No.4 - Just Hatched Spiderling

Images made through a microscope. All subject types.

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Walter Piorkowski
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Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Spiders No.4 - Just Hatched Spiderling

Post by Walter Piorkowski »

Image

Leitz Ortholux microscope
4X Leitz projection eyepiece plus 32:1 relay lens
Olympus 10x Plan Achromat objective.
Image stack, 56 images at 5 micron increments
Reflected light, diffused fiber optic illumination
Canon 50D
Zerene and Photoshop processing.
Expired subject.

This infant spiderling is taking a rest on the exterior surface of the silk spun egg sack it had been hatched from. It is remarkable how nature has provided a means for the spider to fashion silk strands for food acquisition, shelter and a storage cell for its eggs and young.

Walt

rjlittlefield
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Post by rjlittlefield »

What a lovely little creature! I know, my standards are a bit odd sometimes, but I've never seen a freshly hatched spiderling in so much detail. :D

I'm curious about part of the technical description. Apologies if you've explained this elsewhere, but what do you mean by "32:1 relay lens"?

--Rik

Walter Piorkowski
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Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Hi Rik. Seems you still have a soft spot for these little critters.

Getting to your technical question. I have a long winded answer with photos to guide you.

Image

My Ortholux dates back to the early 1960’s. Leitz introduced the 35mm Leica camera to its microscope line at that time through the use of a Micro Camera Attachment. See photo of device off the microscope. The early units came with a 2x conical adaptor that matched the image in the 90 degree eyepiece to the film plane of the Leica camera. This was done with a 1/2x reflex lens that was placed above the prism used by the focusing eyepiece. The attachment came with a 10x Periplan projection eyepiece. I call the reflex lens a relay lens. This camera attachment provides quite a magnification factor.

Image

Image

At some point, my historical records are sketchy, an additional setup was offered with a 1/3x Special Reflex lens. Also called a 32:1 Reflex lens. This attachment had a shorter conical adaptor (see first photo again) and had less magnification which is now a important issue.

I use one of these Micro Camera Attachments on my Ortholux that has the conical adaptor removed. The 10x Periplan has been replaced with a rare 4x projection eyepiece and the lack of a conical adaptor allows me to lower the 50D as close to the 32:1 reflex/relay lens as possible. All this allows me to combat the problem of having too much magnification applied above the objective. All this stuff was made for a 35mm format and with the smaller sensor in my 10D and 50D there is way too much empty magnification. Mr. Charles Krebs has explained all this very well on his web site.

Walt

rjlittlefield
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Post by rjlittlefield »

Walter, thanks for the explanation and pictures. I believe the reason for my confusion is just a typo: "32:1" in your post versus "0.32:1" as engraved on the adapter. It makes complete sense that a 0.32:1 adapter would also be called "1/3x", since 0.32 is pretty close to 1/3.

--Rik

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