Dill stem section

[NileRed]

Hello everybody,

this is my first topic here. I am a chemist (Ph.D. student) and microscopy is just my hobby, which has no (yet) relation to my work. Staining (both fluorescent and common) and pol microscopy are my favorite microscopy areas.

Following photos are stained Dill stem thin sections (water media). Sorry for relatively big thiсkness, I have cut it manually.

First two - AmScope FM320 (Fluor), Canon 550D (ISO 100), A14 CanonSLR, stained with: Quinaldine red (red-yellow color)+Calcofluor white (green), filter set: green (first), red (second).

Last two - Bresser TRM310 (Plan objectives), Canon 550D (ISO 100), A14 CanonSLR, stained with: Toluidine blue-Rhodamine 6G-Acridine Orange (last but one photo) and classical Toluidine blue (last photo).

[elf]

They look nicely done. What does an unstained section look like? How about using a slightly thicker section and doing a focus stacked 3D image? That would really show off the structure.

[NileRed]

Hello Elf,
that is good idea, I guess I will try. Which stacker do you recommend?
I have Helicon Focus and I`v found that quite often people use Zerene (looks more modern), is Zerene generally better?

With best wishes

[elf]

Zerene Stacker has a free trial period, so you can try both to see which works for you best. I've never used Helicon Focus, so can't really say which is better.