Euastrum
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Euastrum
Polarized light, 1/4 wave length plate, retarder (?).
10 X Olympus S plan achromat, Lumipan - Carl Zeiss Jena microscope
- Ernst Hippe
- Posts: 205
- Joined: Sun Aug 16, 2009 9:41 am
- Location: Germany
Hello Ernst,
I could not see any tiny cristals inside the cell, but the flares around it could derive from them. PerhapsThe Olympus 40 X S plan achromat is not enough good to resolve very tine particles. Could you see such cristals inside Euastrum Ernst ? If you look at my another picture - "Cosmarium II" you will be able to see similar flares around the cell. I don't know whet is the primary source of those flares. Do you have any idea ?
I could not see any tiny cristals inside the cell, but the flares around it could derive from them. PerhapsThe Olympus 40 X S plan achromat is not enough good to resolve very tine particles. Could you see such cristals inside Euastrum Ernst ? If you look at my another picture - "Cosmarium II" you will be able to see similar flares around the cell. I don't know whet is the primary source of those flares. Do you have any idea ?
- Ernst Hippe
- Posts: 205
- Joined: Sun Aug 16, 2009 9:41 am
- Location: Germany
NileRed'
I'm not so experienced if about using additional various plates, and I don't know enough to discuss with you. However I can say you that plate I've used was 1/4 lambda (it is engraved on the surface of it). Additionally I've used "retarder" (?). The question mark at the end means that I'm not sure if about the name of that last thing. But I call it "retarder".
Both, the 1/4 lambda and "retarder" I've got lately from my friend and they suits to my Lumipan CZJ microscope
I can not to send the photos of these two plates because I've built them into common housing.
Best regards
Marek
I'm not so experienced if about using additional various plates, and I don't know enough to discuss with you. However I can say you that plate I've used was 1/4 lambda (it is engraved on the surface of it). Additionally I've used "retarder" (?). The question mark at the end means that I'm not sure if about the name of that last thing. But I call it "retarder".
Both, the 1/4 lambda and "retarder" I've got lately from my friend and they suits to my Lumipan CZJ microscope
I can not to send the photos of these two plates because I've built them into common housing.
Best regards
Marek
Marek, I am not also a big specialist in pol microscopy, but if you for example prepare starch suspension in water, make photo at 100 or 400X, with 1/4 and #retarder#, or just #retarder# seaprately (if possible) and send it to me I can help us both to understand what #retarder# mean. All 1/4, 1 or other are named retarder or wave plates, but just #retarder# - God knows, probably 1Lambda, but combination 1+1/4 result in blue background (if I am not mistaken), it is also very important their relative orientation, so combination of 1Lambda with 1/4Lambda may result in yellow, purple or blue background. You can be interested in Michel Levy chart in google! Just enter Michel Levy chart...
With best wishes
With best wishes
- Ernst Hippe
- Posts: 205
- Joined: Sun Aug 16, 2009 9:41 am
- Location: Germany
Ernst,
You are right if about colours in simple polarization. But apart from polarizer and analizer I lately use additionally quarter-wave plate and another "retarder". Their combination, separate rotation and appropriate settings give very various colours. I was also very surprised if about effects which give those three elements. Their combination gives quite different results than simple polarisation - a lot of new colours. And I think it doesn't have to be any crystals to occur those beautiful colours. I think that after my short observations.
Best regards
Marek
You are right if about colours in simple polarization. But apart from polarizer and analizer I lately use additionally quarter-wave plate and another "retarder". Their combination, separate rotation and appropriate settings give very various colours. I was also very surprised if about effects which give those three elements. Their combination gives quite different results than simple polarisation - a lot of new colours. And I think it doesn't have to be any crystals to occur those beautiful colours. I think that after my short observations.
Best regards
Marek
- Ernst Hippe
- Posts: 205
- Joined: Sun Aug 16, 2009 9:41 am
- Location: Germany
Marek,
there is no basic difference between "simple" polarization and such whith a Lambda/4 plate and/or a retarder. It's only a question, how much the colours are shifted up the pol. colours' scale. If something shows dublefraction, it will appear bright or coloured e.g. with crossed pol.-filters. I use retarders myself, also called auxiliary objects, of different path differences.
According to my observations the coloured parts in your crustaceans seem to be only crystals or muscle fibres, let alone tiny sand particles. Please look for specimen whithout pol. colours and compare them.
I'm looking forward to your results and more pictures if possible.
Ernst
there is no basic difference between "simple" polarization and such whith a Lambda/4 plate and/or a retarder. It's only a question, how much the colours are shifted up the pol. colours' scale. If something shows dublefraction, it will appear bright or coloured e.g. with crossed pol.-filters. I use retarders myself, also called auxiliary objects, of different path differences.
According to my observations the coloured parts in your crustaceans seem to be only crystals or muscle fibres, let alone tiny sand particles. Please look for specimen whithout pol. colours and compare them.
I'm looking forward to your results and more pictures if possible.
Ernst
NileRed,
As I promised you, I'm enclosing two pictures presenting my set for photomicrography. This set is not comfortable but it allows taking photomicrographs. As you can see I had to build special construction to my microscope. In the second picture you can see the polarizer and retarders in common housing. If you have got any questions don't hesitate to ask me.
Regards
Marek
As I promised you, I'm enclosing two pictures presenting my set for photomicrography. This set is not comfortable but it allows taking photomicrographs. As you can see I had to build special construction to my microscope. In the second picture you can see the polarizer and retarders in common housing. If you have got any questions don't hesitate to ask me.
Regards
Marek