Moth (Choristoneura Fumiferana) proboscis

Images made through a microscope. All subject types.

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RogelioMoreno
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Moth (Choristoneura Fumiferana) proboscis

Post by RogelioMoreno »

Here is a stacked image of the Choristoneura Fumiferana's proboscis (edge view). 119 frames @ 0.3 microns stacked with Zerene Stacker.

Custom motorized Nikon Labophot, Nikon M Plan Apo 40/0.8, direct projection. For the lighting I used a Nikon 0.85 condenser (bright field).

Image

Rogelio

rjlittlefield
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Re: Moth (Choristoneura Fumiferana) proboscis

Post by rjlittlefield »

Very nice!
RogelioMoreno wrote:119 frames @ 0.3 microns stacked with Zerene Stacker.
0.3 microns may be a new record for PMN. It's kind of daunting to think that even 119 frames gets us only 0.036 mm in focus!

--Rik

RogelioMoreno
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Post by RogelioMoreno »

Rik,

Thank you very much for your comments.

Sorry for my ignorance; but what PMN stand for?

Rogelio

Charles Krebs
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Post by Charles Krebs »

Rogelio,

Have you used any of the 40X M Plans Achromats. If so, I'd be very curious to hear how you think they compare in color correction. (Of course the Apo has a working distance of 0.70mm compared to the much larger working distances of the ELWD and SLWD achromats (10.5mm and 14.9mm) so they really serve different functions).

RogelioMoreno
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Post by RogelioMoreno »

Charles,

I do not have any of the Nikon 40X M Plans Achromats.

I used the M Plan Apo 40/0.8 to test it, I bought it few months ago.

I was looking for a M Plan 40X ELWD or SLWD and found the Apo for $110 (buy now) and could not resist to buy it.

Rogelio

rjlittlefield
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Post by rjlittlefield »

RogelioMoreno wrote:Sorry for my ignorance; but what PMN stand for?
Sorry, my mistake. I was in a big rush and typed without thinking. Somewhere I had recently read a post where PMN was used as an abbreviation for PhotoMacrography.Net. Apparently it lodged in my brain long enough to come out my fingertips again!

I should have just written that "0.3 microns may be a new record for photomacrography.net".

--Rik

Pau
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Re: Moth (Choristoneura Fumiferana) proboscis

Post by Pau »

Excellent image, Rogelio
RogelioMoreno wrote:...119 frames @ 0.3 microns stacked with Zerene Stacker.

Custom motorized Nikon Labophot, Nikon M Plan Apo 40/0.8, direct projection. For the lighting I used a Nikon 0.85 condenser (bright field).
If you show us your setup, it would be very interesting
RogelioMoreno wrote:...and found the Apo for $110 (buy now) and could not resist to buy it.
Good bargain! I would did the same :D
Pau

NikonUser
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Post by NikonUser »

Rogelio: I'm curious how someone in Panama would have an Eastern Spruce Budworm to photograph (an eastern Canada forest pest)
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

RogelioMoreno
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Post by RogelioMoreno »

NU,

I do not know how it arrived here; but maybe the following article could help:

http://bna.birds.cornell.edu/bna/specie ... troduction

Rogelio

NikonUser
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Post by NikonUser »

No!
If it is a local species you have then it is not C.fumiferana but it could be a tortricid; many species are very similar.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

RogelioMoreno
Posts: 2982
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

NU,

Thank you for the information.

Rogelio

Mitch640
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Post by Mitch640 »

So either the worm hitched a ride with the Bay-breasted Warbler, the Bay-breasted Warbler packed it in his lunch, or it flew to Panama on an airline to meet up with the Bay-breasted Warbler. I wonder how it got through security? :twisted:

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

A better argument would be to invoke Occam and his razor :twisted:
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

The image is beautiful. I wonder if surface tension helps draw liquid up that groove? Is the tongue actually hollow, like a straw?

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