I took the first darkfield shot for a general "overall" and then was going to get in really close to look at the tip of a tentacle. When inserting the DIC polarizers it appeared that the tentacle tips contained some particles with birefringent characteristics. So I used a more "overall" objective and went to simple crossed polarizers.
Pretty neat! They lit up like as if they were filled with illuminated glitter. You could watch the "illuminated" particles flow up and down the hydrocaulus. The particles in the tentacles appeared to be fixed in position.



