Polyphemus

Images made through a microscope. All subject types.

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gpmatthews
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Polyphemus

Post by gpmatthews »

Today was the Quekett Microscopical Club excursion to Basingstoke Canal (at Brookwood). A splendid time was had by all, and we enjoyed sorting through a wide selection of species of Cladocera. Here are two specimens of Polyphemus pediculus

The first is probably male, the second a female with brood:

Image
Microscope: Zeiss Standard
Objective: Leitz 16/0.45 NPL Fluotar ICT
Ocular: Zeiss KPL-W 10/18
Substage: Leitz 0.9 NA ICT
Image stack/Zerene Stacker
Sample from Basingstoke Canal, Brookwood, 26-Jun-2010

Image
Microscope: Zeiss Standard
Ocular: Zeiss KPL-W 10/18
Objective: Zeiss Plan 1.6/0.03 - 5/0.1
Brightfield
Sample from Basingstoke Canal (Brookwood) 26-Jun-2010
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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Craig Gerard
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Post by Craig Gerard »

Graham,

The lighting in the first image... Could you expand upon the description of the applied technique?

Craig
To use a classic quote from 'Antz' - "I almost know exactly what I'm doing!"
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gpmatthews
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Post by gpmatthews »

Craig, the first image is a differential interference contrast image (ICT is Leitz-speak for DIC)
Graham

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Craig Gerard
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Post by Craig Gerard »

Graham,

Understood. Thanks.

Craig
To use a classic quote from 'Antz' - "I almost know exactly what I'm doing!"
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