Pleurosigma angulatum (diatom frustule)

Images made through a microscope. All subject types.

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Macro_Cosmos
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Pleurosigma angulatum (diatom frustule)

Post by Macro_Cosmos »

Image

An excellent target for resolution tests! :D

Lou Jost
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Re: Pleurosigma angulatum (diatom frustule)

Post by Lou Jost »

Very beautiful. Can you include technical information?

Macro_Cosmos
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Re: Pleurosigma angulatum (diatom frustule)

Post by Macro_Cosmos »

Lou Jost wrote:
Mon May 10, 2021 11:06 am
Very beautiful. Can you include technical information?
Camera used was a Z6, technique is DIC, at 120x (60x objective with a mag changer's 2x dialled in, so 120x).
No photo-eyepiece, direct projection.
This is a very thin diatom, DIC isn't the best unless it's the high contrast type being used. Phase contrast and COL+POL can yield even better results.

Wemacro for focus stacking as usual, with a focus drive adapted to it (I wrote a post in Equipment Discussions briefing the adaptation).

Lou Jost
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Re: Pleurosigma angulatum (diatom frustule)

Post by Lou Jost »

Thanks very much! NA of the objective?

Macro_Cosmos
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Re: Pleurosigma angulatum (diatom frustule)

Post by Macro_Cosmos »

Lou Jost wrote:
Mon May 10, 2021 11:27 am
Thanks very much! NA of the objective?
NA of the 60x would be 1.2.

Robert Berdan
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Re: Pleurosigma angulatum (diatom frustule)

Post by Robert Berdan »

Beautiful photo!
Cheers
RB

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