Dic prisms are magnification dependant. Oil / dry makes no difference to them.
Ie: i have 1.4 oil DIC condenser with oil dic prisms that work just as well with top lens oiled or dy.
But when i change top lens to 0.9 prisms stop prodicing usable DIC image. I need to change prisms as well.
Search found 465 matches
- Thu Jan 24, 2019 5:58 am
- Forum: Equipment Discussions
- Topic: Oil immersion condenser
- Replies: 25
- Views: 9521
- Wed Jan 23, 2019 5:04 pm
- Forum: Equipment Discussions
- Topic: Oil immersion condenser
- Replies: 25
- Views: 9521
- Wed Jan 23, 2019 8:47 am
- Forum: Equipment Discussions
- Topic: Oil immersion condenser
- Replies: 25
- Views: 9521
- Tue Jan 22, 2019 8:02 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
All my cubes are Nikon original and look like the right one. I know nothing about the left one. I had 4 different versions of that "4mm slider". One was empty, one had polarizer, one had auxiliary barier filter and one was blocking the light. Auxilary barier filters are actually usefull to tune the ...
- Tue Jan 22, 2019 6:35 am
- Forum: Equipment Discussions
- Topic: Judging high-NA infinity-corrected objectives
- Replies: 62
- Views: 20485
In the very near future I will be able to compare the Nikon 20/0.75 with a 2X magnification changer against a native 40/0.75 I do have Microphot with mag changer and some same Na different mag objectives (Finite CFN). 20x/0,75 is effectively replacing 40x/0.75 in my setup (10x/0,45 ; 20x/0,75 ; 40x...
- Fri Jan 18, 2019 7:30 am
- Forum: Equipment Discussions
- Topic: THK KR15 cheap on eBay
- Replies: 78
- Views: 23869
Well, my pair will (hopefully) turn into microscope stage :) I find 30mm travel too small for anything else than micro (mine starts from 10x) JohnyM...same question for you...any update? ...Ray Attempt to adapt them to microscope stage proved to be a disaster. Instead i moved them to XYZ horizontal...
- Wed Jan 16, 2019 8:32 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
- Wed Jan 16, 2019 6:31 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
This is flash test in UV. http://www.photomacrography.net/forum/userpix/3881_Montage_3.jpg 1st row: Full power and 1/2 power 2nd row: 1/4 power and Full power WITH FRESNEL LENS AND FILTER NOT REMOVED I dont have osciloscope to test flash duration. I would say that it matches HBO output (1/1600 sec) ...
- Wed Jan 16, 2019 5:57 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
By the way, if you find useful references such as lists of old cubes/filters/handy fluorophores or equipment documents, I'll put a link in the original post. Send a pm if you prefer. This link that i already posted: https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets ...
- Wed Jan 16, 2019 1:27 am
- Forum: Equipment Discussions
- Topic: Which camera bodies have fully electronic shutter?
- Replies: 39
- Views: 12752
It seems to be explained quite well here:
https://www.dpreview.com/articles/58166 ... -to-know/2
Makes sense why Olympus allows flash at very long exposure times.
I guess that with A9II we'll get flash and electronic shutter.
https://www.dpreview.com/articles/58166 ... -to-know/2
Makes sense why Olympus allows flash at very long exposure times.
I guess that with A9II we'll get flash and electronic shutter.
- Tue Jan 15, 2019 8:48 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
I notice the B cube doesn't have at least one filter. That makes sense if they're using the mirror to cut all the short wavelengths. I'll get the cubes together and see what comes out of their colours.. No, that's very bad. No mirror - obviously wont work. No barrier - there will be bleeding. No ex...
- Tue Jan 15, 2019 8:45 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
- Tue Jan 15, 2019 8:38 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
1)On the flash - is that a Nikon fitting? Is the UV filter removed from the flash, or is there just so much light anyway that it's not necessary, or using Wood's glass, or......? 2)Which model 'scope is it - maybe one day? :) 3)I just dismantled an old Sony 3MP camera, only keeping the screws. But ...
- Tue Jan 15, 2019 7:25 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
Cube Excitation Emmision DM580 (BA590) G-2A / G-1A Green Red only DM510 (BA520-560) B-1E / B-2E Blue Green only UV-2A BA420 DM400 UV-2A UV 365nm All the colors B Most common B cube is B-2A You can unscrew excitation filters, you'll be able to read the marking then and can tell for sure which cube yo...
- Tue Jan 15, 2019 7:13 am
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13672
This webpage is quite good: https://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets Rule of thumb: -Amateur would like autofluorescence (many sparkling colors) so gravitate towards left side of the spectrum and wideband (excitation) longpass (emission) cubes. Multiband cub...