Great images Charlie! Nice feeling of depth in the copepods.
Nice chloroplasts in the last image, is that some Stephanodiscus?

Best wishes, René

myriophyllum wrote:Hi René,

The diatom was in water, so all the light should normally be removed by total reflexion on top of the glas slide (a common 1mm one).

Ah, I forgot about that. And now I get your remark about TIRF as well.
No, I guess this is just magic at the edge of it's aperture ;-)

René

Thank YOU Bruce!

Thank you guys! I was hoping for a positive identification Bruce
Thanks for the effort on stacking btshumy. I'm always a bit disappointed by my own efforts on stacking brightfield images, but your image does give a good first-view feeling.

René

Hi Jens, did you play with the condenser height and found a position with darkground illumination? It's probably not fully corrected and you found a spot where the most oblique rays are separated (due to remnant sperical abberation)

Best wishes, René

From an experiment a couple of years ago: typical ciliates from a brackish envirioment, possibly Diophrys. Lugol preservation, dehydrated in ethanol and mounted in Euparal. Size ca. 85um, DIC, 20x/0.7 splanapo. Best wishes, René http://www.photomacrography.net/forum/userpix/154_Uronychidae_17_102634...

Nice result Jens! No, it has nothing to do with TIRF, with that you wouldn't be looking at the skeleton, but at the (fluorescence from the) membrane around the diatom cell.

Best wishes, René

Sure Fan, I've shown some details here on Frustulia: http://www.microscopy-uk.org.uk/mag/artapr09/rvw-contrast.html . Both phase and COL images were made with a Heine condenser. Not too bad I would say. The crossed-polar method on Frustulia went really weird, I remember. Pity I didn't include an ima...

Wow! Looking forward to further postings this year!

René

Thanks for the comments guys. I realize it can be hard to make sense of the structure. In fact it's a smaller version of Podosira stelliger, which I showed here before: http://www.photomacrography.net/forum/viewtopic.php?t=15139 . There are no solid differences between these two genera and they will...

Got some samples in from a UK harbour this year (Hull) last year which got some of those typical Podosira/Hyalodiscus species. So I decided to spend some time preparing it in different orientations. The valve diameter of this marine diatom is 42um. So valve upwards, sideways, girdle and valve downwa...

Hi Walter, best wishes for 2017 to you too!
The first one is Lithodesmium undulatum, the 6th one is the sideview of Biddulphia alternans. The 3th I am not too sure, but certainly not Rhaphoneis.

Good luck, Rene

mmhm, defenitely something wrong with that bottle. It should be touch dry within half an hour, that´s the reason for it´s succes in hospital usage!
You can still soak of the coverslip and remount it.

Best wishes, René

Beauties again Charles, thanks for that!
For some reason I always seem to see it from the inside. Nevertheless an impressive experience.

Best wishes, René

Absolutely, splendid image! Distephanus/Dictyocha speculum.

Best wishes, René