Hello, I’ve been noticing that my daylight whitebalance setting is pretty close, but not perfect. The background appears a little grey. What is the correct way to whitebalance and meter a brightfield scene? Should I aim to blow out the background or should I attempt to get the background perfectly ...

Hello, I’ve been noticing that my daylight whitebalance setting is pretty close, but not perfect. The background appears a little grey. What is the correct way to whitebalance and meter a brightfield scene? Should I aim to blow out the background or should I attempt to get the background perfectly w...

First you need to be sure if the sensor is too low or too high. No worries. I fixed the parfocal issue by adjusting my eyepieces on the ergo head. Despite not having experience with this equipment, to me the coupling between both tubes doesn't seem right (red arrow), did you refer to filing it in y...

I'm pretty happy with this setup at this point. My only complaint is that the image circle in the camera is a pretty small crop of what I see through the eyepieces. I may try 2.0x PLI eyepiece in the future. At first, I thought the image through live view was a bit odd. It looked washed out. But the...

Here's a photo of the TMS-F f mount adapter bottomed out in the V-T photo tube: https://i.imgur.com/DWVKaZ9l.jpg You need to adjust parfocallity at the seat of the 2.5x lens, not by cutting off the black tube. If it's not off by much, and both eyepieces are adjustable, you may be able to make both o...

TMS-F f mount adapter came in. It fits and works in FX mode, though it isn't parfocal. The f mount adapter's black pipe sticks up a bit from the top of the V-T photo tube. Probably if I saw the end off with a horizontal bandsaw it'll be closer to parfocal. I'll probably try that next. For now, here'...

Getting max FOV out of your D850, you should try the (V-T adapter MAB53410)+(PLI 2.5x relay lens)+(TMS-F F mount adapter tube) The combination will project an 18mm diameter field magnified 2.5x to the sensor plane. That Lmscope thing is way too expensive... The TMS-F F mount adapter tube is obsolet...

Another option is to use direct projection but use a teleconverter at the camera I have the Nikon AF-S FX TC-14E III (1.4x) Teleconverter. Should be an interesting experiment if I end up with a DX direct projection setup. Having it at hand, test direct projection with it over the Y-IDP photoport. L...

Have you tried removing the short tube? Yes, I can get it mostly in focus, but the grip and nose of the camera body get in the way, so the camera body is tilted a bit, which isn't ideal for focus. 10x APO ISO 500 1/50 FX: https://i.imgur.com/MA1neop.jpg 10x APO ISO 500 1/50 DX: https://i.imgur.com/...

I'd like to give direct projection a try. However, I need to know the distance between the trinocular port and the top of the f-mount so I can fabricate an adapter. Does anyone either have these measurements available or know how I can figure them out? UPDATE: Unfortunately direct projection won't w...

One weird thing I've noticed is that my stage drifts to the right sometimes. I won't be touching anything and I'll notice the image slowly scrolling to the right. It does this for a few seconds and then stops. What could be causing this? I've also noticed a bit of play when I touch the stage movemen...

Because you are inexperienced to begin I recommend you two booklets available to download from Zeiss and other internet sites: "Microscopy from the very beginning" Thanks for this. Very helpful. I now feel like I have a fairly decent grasp on Kohler illumination. Image quality has improved quite a ...

Aside the risk of damage due to the small WD there is an optical factor: your objectives have written on them /0.17 because the cover is part of the optical system, as you suggest due to the different RI of glass and air. Without cover you'll get spherical aberration degrading the image, it scales ...

Pau wrote: And be aware that your biological microscope is designed for transparent samples mounted with a 0.17mm cover glass. Only the 4X and in some cases the 10X are adequate for uncovered specimens.

Ok, but why? Is that a risk of damage thing or a refractive index thing or what?

Parfocality between objectives in a microscope usually is not perfect, requiring a bit of refocusing with the fine focus knob is normal, but if you do not see an image even defocused when changing to the next magnification you have a parfocality issue. I count three and a half turns of the fine foc...