Search found 26 matches

by dlg
Sun Nov 08, 2015 11:30 am
Forum: General Discussion Forum and Community Announcements
Topic: Printing
Replies: 4
Views: 2580

I print once in a while, mainly 13x19", on epson 4880 with epson paper. I have a few hanging at home in a "changing gallery" The rest is in boxes. I also make greeting cards and use those once in a while. Some of the 13x19 images were used in a recent exhibit at my museum. Here are a couple of image...
by dlg
Sun Nov 08, 2015 11:14 am
Forum: Equipment Discussions
Topic: Zeiss Axioskop2plus epi for z-stacking?
Replies: 11
Views: 1444

Thanks to all three of you to the various indications. the NA 0.3 for coverslips is very helpful. 4th power function is fully appreciated. The 5x/0.16 ICS/0.17 planapo has a WD of 12 mm, so should work just fine. the 10x/0.3 ICS/0.17 planapo has a WD of 2 mm, so too tight. There is an EC Neofluar 10...
by dlg
Sun Nov 08, 2015 10:31 am
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

Re double stain, each stain has its own specificity. Don't recall the details on Toluene vs Methylene blue. It is a good general histo stain that also works on plastic embedded material. Not sure about H&E, which has a strong red component anyway. Re fabric dies, why not? You can also put the flower...
by dlg
Sat Nov 07, 2015 8:26 pm
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

Lou: I sure know about paperwork! Just offering to lend a hand. Flowers prepared for SEM are not "destroyed" at all. They are simply dried, and now attached to a SEM stub. I have all my own flower samples and all my Kew preps available for re-examination. I think you could over-stain the flowers and...
by dlg
Fri Nov 06, 2015 11:05 pm
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

If you need a few SEMs, and can get the preserved flowers into the US, I can take some SEMs for you. Send me an e-mail directly for details. Re EtOH specimens, they all go in the SEM, sorry. However, any standard stain such as carmine red or methylene blue should do the trick. This should give you s...
by dlg
Fri Nov 06, 2015 10:26 pm
Forum: Equipment Discussions
Topic: Zeiss Axioskop2plus epi for z-stacking?
Replies: 11
Views: 1444

Zeiss Axioskop2plus epi for z-stacking?

I currently take images of small (1-3 mm) flowers with Canon MPE-65 and stack shot (resolution limit around 10 µm), or on a Zeiss Discovery V20 mot with planapo 1.5x (resolution limit around 4–6 µm). I've been toying with the idea of using my compound for z-stacking (resolution to 1.6 µm with NA 0.4...
by dlg
Fri Nov 06, 2015 6:41 pm
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

Have you thought about SEM for your flowers? You will need critical point dryer. I tried it with hexamethyldisilizane, but does not work even with 2 week incubation. That will be part of a ms I just submitted to AOS-ORCHIDS/Lindleyana section. The cell surface sculpture you get with SEM is simply un...
by dlg
Fri Nov 06, 2015 1:15 pm
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

I look forward to hearing about your orchid photography experiments and techniques. Tiny orchids are also my specialty (pleurothallids particularly). Hi Lou, pleuros are a lot of fun, too. If you want, you can check out Geiger, D. L. 2013. Imaging small orchid flowers using visible light. Orchid Di...
by dlg
Thu Nov 05, 2015 11:13 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: 5DsR/MPE65 useful magnification experiments
Replies: 8
Views: 4849

dlg, welcome aboard here at photomacrography.net. :D For the benefit of other readers, let me provide some further analysis that was contained in that personal e-mail you mentioned. BTW, I noticed in your posting [ at CanonRumors ] some discussion as follows: the question arises at what magnificati...
by dlg
Thu Nov 05, 2015 9:26 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: 5DsR/MPE65 useful magnification experiments
Replies: 8
Views: 4849

5DsR/MPE65 useful magnification experiments

First serious post here. I got the new Canon 5DsR, 50 MP full frame sensor :-) I posted this on Canonrumors, but thought some of you here may also be interested. I've been playing around with it to see how it behaves in the macro area. I shot some small orchid flowers (Oberonia cf. lunata), total he...
by dlg
Thu Nov 05, 2015 7:46 am
Forum: Community Members and Friends
Topic: macro, stereo, compound, SEM natural history guy from So Cal
Replies: 16
Views: 6218

macro, stereo, compound, SEM natural history guy from So Cal

Finally joined here, after a bit of renewed interest in natural history imaging of mainly <1-5 mm shells and orchid flowers. Use a range of equipment from 5DsR with Zeiss 100MP/ZE, MPE65, stackshot, to Zeiss Discovery V20 mot with planapos 0.63 and 1.5x and Axiocam HRc, Axioskop IIplus with set of p...