Nice set, interesting setup with the led. My favourite is the last one.


Regards,

Walter

Nice catch and good darkfield, well done!

Regards,

Walter

Thanks for sharing these excellent pictures, lovely colour and detail.

You cut them without fixation on a vibratome? I suppose the tissue was strong enough to be sectioned?

Nice set and interesting subject, thanks for sharing.



Regards,

Walter

Nice and very colourful set, thanks for sharing!

Regards,

Walter

Hi Robert,

Very interesting processing technique, thanks for sharing.
Previously I've also experimented with " deconvolution like" technique by merging translucent layers of red and black in Photoshop, (chlorophyl fluorescence).

The 104 Forms type slide created by Klaus Kemp is still with me and I found the opportunity to experiment a bit more with darkfield, an 100x iris objective and (circular) oblique light. After a lot of fiddling around I found a setup that provided images with detail as well as a more 3-dimensional ap...

Beautiful set with many interesting details.

Thanks for sharing!

Regards,

Walter

Hi Charles, thanks for your positive response! Nice set! I'm partial to the darkfield myself, very 3-dimensional and great light light on the upper surface (is the body somewhat translucent?) The body is a rather translucent indeed, small insects are therefore good to combine with darkfield. I do li...

Beautiful set with many interesting details. Many thanks for sharing!


Regards,

Walter

Hi Pau and Saul, thanks for your positive remarks! @Pau; yes correct it is an afocal setup, identical to the setup with the Orthoplan I posted a couple of years ago in equipment discussions. The camera lens is indeed a pancake and the camera is hanging on the ceiling with a beamer support, so differ...

Beautiful set!
Thanks for sharing.



Regards,

Walter

Thanks both for your positive remarks. @Houstonx: below a couple of pictures of my DIC -indeed ICT- setup. Center is an Ortholux II ( the "new" Ortholux) with an eyepiece head designed for 30mm diameter eyepieces. This results in a field number of 24, where the standard FN is 18. So it catches a lar...

Nice set, thanks for sharing!

Regards,

Walter

Nice catch, thanks for sharing.

Regards,

Walter