Search found 4861 matches

by Pau
Mon Aug 02, 2021 9:52 am
Forum: Photography Through the Microscope
Topic: diatom arrangement type slide, Dunkirk, MD
Replies: 2
Views: 64

Re: diatom arrangement type slide, Dunkirk, MD

Wow! excellent slide, admirable mounting skills ...As I found an identical one without the deformed "arm" (left side, third row) I think it is a mutation of the same species. Nice find, although IMO with only one deformed specimen and without genetic sequencing you can't propose a mutation; the phen...
by Pau
Sun Aug 01, 2021 10:45 am
Forum: Photography Through the Microscope
Topic: Collagen
Replies: 1
Views: 59

Re: Collagen

Very beautiful.
The striated muscle cells birrefringence is also very well shown
by Pau
Thu Jul 29, 2021 6:38 am
Forum: Equipment Discussions
Topic: A7r4 pixel shift. Rather a resolution eye-opener
Replies: 31
Views: 1338

Re: A7r4 pixel shift. Rather a resolution eye-opener

rjlittlefield wrote:
Wed Jul 28, 2021 10:23 pm
I get a "404 page not found" error on the second one.
I get the same, check if it is password protected
by Pau
Tue Jul 20, 2021 3:14 pm
Forum: Equipment Discussions
Topic: 20X Objective Quest
Replies: 9
Views: 345

Re: 20X Objective Quest

Another good one is the Olympus LMPlanFl 20/0.40, small but visible longitudinal CA and high quality field smaller tnan the mitu but very good for APSC
by Pau
Sun Jul 18, 2021 1:16 am
Forum: Photography Through the Microscope
Topic: Diatom Arrangement with (Heine) Phase Contrast
Replies: 4
Views: 179

Re: Diatom Arrangement with (Heine) Phase Contrast

Very nice phase.
I still prefer your delicate BF images. A DIC version would be nice to complete the comparison.
by Pau
Fri Jul 16, 2021 3:56 am
Forum: Photography Through the Microscope
Topic: Silicate for the pleasure of eyes
Replies: 3
Views: 147

Re: Silicate for the pleasure of eyes

Beautiful.
What silicate? could it be Muscovite or another mica?
by Pau
Fri Jul 16, 2021 3:50 am
Forum: General Discussion Forum and Community Announcements
Topic: I need advice to get my money back
Replies: 13
Views: 3293

Re: I need advice to get my money back

Fero wrote:
Fri Jul 16, 2021 3:26 am
and btw microscopic items are free of duties in EU (not VAT though)
In Spain we pay both duties and VAT, microscope items have not privileges :cry:
by Pau
Fri Jul 09, 2021 2:04 am
Forum: Photography Through the Microscope
Topic: Seaweeds fluorescence
Replies: 12
Views: 363

Re: Seaweeds fluorescence

Thanks Macro_Cosmos, Didier_Micro and ModelZ for your comments.

I didn't see that humble alga diabolic until your comment :lol:
by Pau
Fri Jul 09, 2021 1:57 am
Forum: Photography Through the Microscope
Topic: Amphipleura pellucida
Replies: 2
Views: 153

Re: Amphipleura pellucida

Excellent test, the classic subject beautifully resolved
1µm / 4 = 0.25µm very near the Abbe's limit
by Pau
Fri Jul 09, 2021 1:49 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: June Beetle eyes have a smoothly curved surface
Replies: 9
Views: 350

Re: June Beetle eyes have a smoothly curved surface

The stereos are not just nice but fully adequate to show the structure and to illustrate the concept =D>
by Pau
Mon Jul 05, 2021 2:32 pm
Forum: Photography Through the Microscope
Topic: Seaweeds fluorescence
Replies: 12
Views: 363

Re: Seaweeds fluorescence

Acetabularia mediterranea , for me the most beautiful alga, an umbrella shaped giant unicellular Chlorophyta alga measuring up to several centimeters. This one is a small sprcimen, about 7mm diameter.
2021-07-05-23.10CorrBR.jpg
UV 365nm with external torch, old Leitz Plan 2.5/0.08, short stack
by Pau
Mon Jul 05, 2021 11:11 am
Forum: Equipment Discussions
Topic: episcopic adapter
Replies: 22
Views: 745

Re: episcopic adapter

Sorry, I'm not at all sure of fully understanding your ideas, as you likely know my Maths and Optics knowledge is less than basic. With my fluorescence epi-illuminator I can see the LED image focused at a plane that I assume is the rear focal plane in a window exactly designed for this purpose (sorr...
by Pau
Mon Jul 05, 2021 10:07 am
Forum: Photography Through the Microscope
Topic: Seaweeds fluorescence
Replies: 12
Views: 363

Re: Seaweeds fluorescence

Thanks ADi, Luis Carlos and Lou! How are you dealing with quenching and bleaching? In the Rhodophyta yellow emission bleaching is negligible, at least during a 10min set up and stack session, but as you well know chlorophyll photobleaching is important and annoying. In this cases algae bleaching was...
by Pau
Mon Jul 05, 2021 9:48 am
Forum: Equipment Discussions
Topic: episcopic adapter
Replies: 22
Views: 745

Re: episcopic adapter

To be more exact, the "epicondenser"* optics must focus the light source at the objective rear focal plane and fulfilling it, like in transmitted Köhler

* this is the usual name although the main condenser in this case is the objective itself
by Pau
Mon Jul 05, 2021 7:21 am
Forum: Equipment Discussions
Topic: episcopic adapter
Replies: 22
Views: 745

Re: episcopic adapter

I have tested it only with the x4, that is not the best objective for this use. Sure, at least in general it would be more flare prone than higher mag. ones, but because the objective is also acting as condenser the visual or camera sensor coverage can be pretty similar (being the actual illuminate...