Search found 4721 matches

by Pau
Fri Jan 22, 2021 4:25 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Auto focussing by moving the camera
Replies: 2
Views: 27

Re: Auto focussing by moving the camera

I have dreamed with such a system, both for autofocus and autostacking. :-k Theoretically this could be done if you take the focusing electric signals from the objective bayonet camera mount and feed with them a motor similar to the lens focus one but driving the rail. But usually rail motors are mu...
by Pau
Mon Jan 18, 2021 4:16 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Advice please: High NA air objective with small WD
Replies: 15
Views: 3769

Re: Advice please: High NA air objective with small WD

Question: Do I need to collimate the light source? I was planning to experiment to find out, but you likely know the answer already. As usual in Microscopy the ideal illumination is after Kölher: focusing the light source at the objective rear focal plane filling it. This is what epiilluminators do...
by Pau
Sat Jan 16, 2021 1:16 am
Forum: Equipment Discussions
Topic: 50X objective recommendation
Replies: 31
Views: 1105

Re: 50X objective recommendation

Wow - what kind of rail are you using to reach ~250 nm steps? That is closer to linear servo territory. You can get step sizes almost that size with simple, cheap off-the-shelf microscope focusing block driven by the cheap WeMacro MicroMate. This is what I do, depending of the microscope focus bloc...
by Pau
Sun Jan 10, 2021 6:13 am
Forum: Equipment Discussions
Topic: Monochromatic Microscopes?
Replies: 21
Views: 837

Re: Monochromatic Microscopes?

I am a little surprised at the low NA. .. ... I am surprised that the NA would go backwards as technology advances. Well, what matters is resolution, not NA itself. The former generation with immersion optics had 1.35 NA and lower resolution, the key as you well know is the shorter wavelength, and ...
by Pau
Fri Jan 08, 2021 11:52 am
Forum: Equipment Discussions
Topic: Raynox dcr150 custom tube lens
Replies: 21
Views: 807

Re: Raynox dcr150 custom tube lens

Don't forget to put anti-reflection flocking inside to avoid glare
by Pau
Fri Jan 08, 2021 2:34 am
Forum: Beginners Micro
Topic: Olympus BH-2 Camera adapter
Replies: 6
Views: 337

Re: Olympus BH-2 Camera adapter

As others said this is a very nice microscope stand. But beware: the main part of a microscope is the objective(s). The BH is designed to work with DIN objectives (45mm parfocal and 160mm tube corrected). In your picture I see what seems a Olympus 160 objective together with a Zeiss infinite correct...
by Pau
Fri Jan 08, 2021 2:15 am
Forum: Photography Through the Microscope
Topic: Sea Urchin Spines SEM
Replies: 7
Views: 390

Re: Sea Urchin Spines SEM

Very nice image, I love SEM (and have no access to one of them) They were the typical purple and white urchin color before sample prep. Still trying to figure out how to add the color back in... I would try the following approach (just a not proven idea): after setting up the sample but before metal...
by Pau
Wed Jan 06, 2021 4:23 am
Forum: Equipment Discussions
Topic: Raynox dcr150 custom tube lens
Replies: 21
Views: 807

Re: Raynox dcr150 custom tube lens

Just to point that the term tube lens in our context refers to the lens itself (in your case the Raynox) and not to the tube that contains it. A is the distance that focus the tube lens alone (without objective) to infinite. With low magnification infinite objectives you can play with it to some ext...
by Pau
Wed Jan 06, 2021 2:41 am
Forum: Equipment Discussions
Topic: Monochromatic Microscopes?
Replies: 21
Views: 837

Re: Monochromatic Microscopes?

There are also economical, extremely good 5x and 10x monochromatic "objectives" with high NA (greater than 0.35 in many cases), zero distortion, perfectly flat fields, and enormous image circles. These are among the best optics in existence, as long as you use the right wavelength of light. They ar...
by Pau
Mon Jan 04, 2021 3:25 pm
Forum: Equipment Discussions
Topic: Monochromatic Microscopes?
Replies: 21
Views: 837

Re: Monochromatic Microscopes?

Well, any microscope is monochromatic if you use monochromatic light.

A green filter has been a standard accesory with most new microscopes for long time for the very same reason you suggest
by Pau
Sat Jan 02, 2021 10:09 am
Forum: Equipment Discussions
Topic: New 1.4x Lens Test Results Online
Replies: 24
Views: 800

Re: New 1.4x Lens Test Results Online

Excellent tests again, thanks for posting them.

Note: PM sent
by Pau
Fri Jan 01, 2021 8:42 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: My MacroScope Project
Replies: 4
Views: 469

Re: My MacroScope Project

Ray, thank you!
by Pau
Fri Jan 01, 2021 2:20 am
Forum: Equipment Discussions
Topic: microscope recommendations
Replies: 12
Views: 609

Re: microscope recommendations

Oil darkfield condensers are crazy expensive. Dry ones are even rarer. :oops: Zeiss dedicated dark field condensers (two dry models and one oil "ultra condenser") are both excellent and relatively abundant at the used market. You would need to make or order a dovetail adapter, Charles Krebs did it ...
by Pau
Fri Jan 01, 2021 2:15 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: My MacroScope Project
Replies: 4
Views: 469

Re: My MacroScope Project

... The one drawback I found to the Pentax bellows was the focus rail clamp is the weak point in the system, and it caused me a lot of trouble with flexibility and slow recovery times to external vibration. In my own system today I have eliminated the clamp and mounted two focus rail X extrusions t...
by Pau
Tue Dec 29, 2020 12:29 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Questions about using a YN568EX III flash unit for photomicroscopy
Replies: 4
Views: 336

Re: Questions about using a YN568EX III flash unit for photomicroscopy

I use a YN565EX and a Canon 7D, a different but comparable system. After some attempts I got it working as slave (I don't remember the settings) but anyway I found much more convenient a hot shoe cord exactly as suggested by Rik. ETTL-2 is very useful in general photography but I find that manual mu...