Yes, macro - but very striking, anyway!

I've struggled with it too, which is one of the reasons I tend not to do stacking with these images. I've seen it too - to do with the thickness of the diatom? Only occurs with some. Louise I'm not sure why it happens, but looking at my image above where it is obvious, it seems to occur at the edge...

During UV photoraphy, I also meet this phenomenon on the edge of diatoms, I think it's artifact, but don't know how to improve it, focus stacking does not work. I've struggled with it too, which is one of the reasons I tend not to do stacking with these images. I've seen it too - to do with the thi...

My camera does have EFSC, I think you are getting mixed up with the D750 and the D7500 haha. I’m not sure if I wrote a typo somewhere but I’m using the D7500. I’ll try connecting it to a laptop, but I can tell on my live view screen that the image is sharper than the resulting photo so it is defini...

Hi I would try with the camera tethered. That ensures the mirror is locked up and a larger screen makes it easier to check how well the camera sensor is focused at the time of exposure. If you have a clear, sharp image on your PC/laptop screen then you should expect that to be the same in the saved...

Hi I would try with the camera tethered. That ensures the mirror is locked up and a larger screen makes it easier to check how well the camera sensor is focused at the time of exposure. If you have a clear, sharp image on your PC/laptop screen then you should expect that to be the same in the saved ...

Hi

Are you using the D750 in liveview mode and tethered to a PC/laptop? That's how I use my Canon and avoid camera/mirror vibration which otherwise spoils images. Also, you should be able to adjust your trinocular port / sensor distance to get parfocality with the oculars.

Louise

Sadly for all who knew Klaus Kemp, either through personal contact or as an admirer of his work. Received the news that he passed away on the 28th May 2022 in Hospital. In his passing, the world of micro-manipulation has lost a great master of the art. R.I.P Sad. https://www.youtube.com/watch?v=DwE...

Another possible factor: at same NA, the light cones at the subject will be narrower, in proportion to the RI of the immersion fluid. So where NA 0.95 requires light rays that are angled at 71.8 degrees off-axis, NA 1.0 in RI 1.51 requires only 41.5 degrees off axis. --Rik Interesting, Rik. Do you ...

If nothing else there is less scattering/reflections because of the lack of air interfaces when the immersion fluid is used. Another possible factor: at same NA, the light cones at the subject will be narrower, in proportion to the RI of the immersion fluid. So where NA 0.95 requires light rays tha...

I have the same Nikon 100x - I must have another play with it too! I get distracted too easily but will try and have another go with it sometime this week. I did get some borosilicate coverslips from Laboquip https://londonlaboquip.com/product/laboquip-microscope-coverslips-18x18mm-glass/ which hav...

I have the same Nikon 100x - I must have another play with it too! I get distracted too easily but will try and have another go with it sometime this week. I did get some borosilicate coverslips from Laboquip https://londonlaboquip.com/product/laboquip-microscope-coverslips-18x18mm-glass/ which have...

The objective bought with RMS thread is PLAN NEOFLUAR 2.5X / 0.075 ∞ / - The microscope I have is a phase contrast microscope, brand Amscope: https://amscope.com/products/t610-ipl-ph Subsequently (about 8 months ago) I bought the following objectives: 4X, 10X, 20X and 40X from the following link: h...

Awesome! How did you do it, exactly? Louise Thank you, Louise! The workflow is as follows: 1. I thresholded confocal stack and made it binary in Fiji/ImageJ. Opened it in 3DViewer plugin as surfaces, exported the mesh as an .stl file. 2. cleaned up the mesh in Blender - filled in all the holes, sym...

Awesome! How did you do it, exactly?

Louise