Search found 146 matches

by jmc
Sat May 07, 2022 9:36 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

That's better than I had expected! Thanks for doing these tests. I wonder what the image quality is like. Did you do these tests with glycerine immersion? I wouldn't expect it to matter much, but the internal reflection off the front surface of the front lens would be reduced if it were immersed in...
by jmc
Sat May 07, 2022 8:21 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Great, I very much look forward to knowing the results! Thank you. I hope it at least has some useful properties, even without deep transmission. The Nikon UV-F 100x NA 1.3 arrived (along with a 10x Fluor NA 0.5 as well) so I measured the transmission of them in the UV. The Nikon UV-F 100x NA 1.3 i...
by jmc
Fri May 06, 2022 6:53 am
Forum: Photography Through the Microscope
Topic: Diatoms in Circular Oblique Light Update 06/05
Replies: 26
Views: 4701

Re: Diatoms in Circular Oblique Light Update 06/05

I know this is a couple of years old now, but what amazing images. Thanks for sharing.
by jmc
Wed May 04, 2022 6:59 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Jonathan, there is a whole series of UV-F objectives (10x, 20x, 40x, 100x), all glycerine immersion objectives. Fluor objectives have always been around and continue to be made, and are completely independemt of this UV-F line. The 40x UV-F has an NA of 1.30, one of the highest NAs for such a low p...
by jmc
Wed May 04, 2022 5:36 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Thanks all. Scarodactyl, in the Nikon CF objectives brochure the UV-F 100x is included with the Fluor ones in the Epi fluorescence section - Featuring extra-high light transmission into the ultraviolet excitation range the high NA fluor dry and fluor oil objectives are ideal for observation and phot...
by jmc
Tue May 03, 2022 2:35 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

I have the Nikon version of this (160mm tube length "UV-F" 100x 1.20) but have never tried it. This result makes me want to try! Lou. I managed to find one of these UV-F 100x objectives for sale in Europe and have bought it. When it arrives, I'll do some testing on it and let you know how I get on....
by jmc
Sat Apr 30, 2022 1:50 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Thanks, I think you are right about the Nikon UV-F series being aimed at fluorescence rather than deep UV. Too bad. No problem Lou. If you have a suitable light source, camera and filters for imaging at 365nm then it is probably still worth a try at that wavelength. But I wouldn't bother if you nee...
by jmc
Fri Apr 29, 2022 2:51 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

I have the Nikon version of this (160mm tube length "UV-F" 100x 1.20) but have never tried it. This result makes me want to try! Hi Lou, I believe (though I admit, I have never tested) that the Nikon UV-F 100x will have different UV transmission to the Leitz UV I was using. Let me explain. The Leit...
by jmc
Thu Apr 28, 2022 11:10 am
Forum: Equipment Discussions
Topic: Help identifying 'Astro' microscope objective please
Replies: 0
Views: 74

Help identifying 'Astro' microscope objective please

I need a bit of help identifying a mystery objective please. The person I got it from knew nothing about (it was from ebay from a vendor who dealt in general second hand stuff, but wasn't a microscope dealer). The mount seems to be the same diameter as RMS but is a finer pitched thread. I could get ...
by jmc
Thu Apr 28, 2022 11:01 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Well done! Quite dramatic to see the resolution improving. BTW, are you able to make an image with a normal 100x oil immersion objective? I expect that to give a better image than such a specialized UV objective in the normal light region. Best wishes, René Thanks René, yes it was really nice to se...
by jmc
Wed Apr 27, 2022 1:36 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

Minor update. I got a Newport Highres-2 USAF test slide to help with my work - https://www.newport.com/f/high-resolution-test-targets The one I have is a positive one, with opaque lines on a clear quartz slide. I've done some resolution testing at different wavelengths with that Leitz 100x NA 1.2 UV...
by jmc
Thu Apr 21, 2022 3:51 am
Forum: Administrator's Appreciation Gallery...Photography Through the Microscope
Topic: Radiolaria
Replies: 12
Views: 872

Re: Radiolaria

Wow, beautiful images.
by jmc
Tue Mar 22, 2022 3:18 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

I'm not at all convinced by this report, typical tunnelvision of a couple of material scientists. But indeed Lou, instead of using a high refractive mountant to create contrast (which doesn't work in UV in any case), you can use water and still be able to generate (absorbance) contrast. As usual fo...
by jmc
Mon Mar 21, 2022 6:12 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

I just found this interesting article suggesting that diatom frustules interact differently with UV light versus visible light. This might make UV light more effective for photography than we might expect by the wavelength-resolution advantage? https://www.nature.com/articles/s41598-018-21810-2 Int...
by jmc
Sat Mar 19, 2022 8:40 am
Forum: Photography Through the Microscope
Topic: Diatom imaged with 313nm light
Replies: 32
Views: 1541

Re: Diatom imaged with 313nm light

I just found this interesting article suggesting that diatom frustules interact differently with UV light versus visible light. This might make UV light more effective for photography than we might expect by the wavelength-resolution advantage? https://www.nature.com/articles/s41598-018-21810-2 Ooo...