Search found 223 matches

by kds315*
Fri May 29, 2020 3:31 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

So Lou, when you say "I would like to use through-the-objective UV lighting to excite fluorescence." this gives you much more choice in terms of objective lenses, and you wouldn't need to ultra expensive ones designed for imaging in the UV. As mentioned above the Olympus UV Fl ones are worth lookin...
by kds315*
Fri May 29, 2020 3:27 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

Lou Jost wrote:Thanks for that advice. I have a Nightcore UV flashlight which I think uses a Nichia LED...
Their drivers often are switching, so I'm not sure those are flicker free.
by kds315*
Thu May 28, 2020 5:53 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

Peter Höbel http://www.mikroskopie-ph.de/ occasionally shows UV images of diatoms for increased resolution in the German forum. He uses a 365 nm LED, oblique illumination, a selection of finite objectives and a monochrome C-mount camera. High intensity UV and long aquisition times are quite incompa...
by kds315*
Thu May 28, 2020 5:32 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

Thanks Klaus. I had seen those UV Lomo objectives. On this forum we've seen a very wide variance in the quality of different Lomo objectives, from "best in class" (3.7x) to unusable. Is there a Lomo UV that is really good? Can really say, I have a few but packed away deeply and I haven't really mad...
by kds315*
Thu May 28, 2020 5:23 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

Klaus, thanks, I know the Ultrafluar is good, but very expensive. Are the other ones you mentioned really good? For both visible and UV light, or just UV? I would like to use through-the-objective UV lighting to excite fluorescence. And why do so many UV objectives require a coverslip? Doesn't a co...
by kds315*
Thu May 28, 2020 3:15 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Is anyone doing UV transmission microscopy?
Replies: 33
Views: 1539

Don't forget the really excellent full color corrected ZEISS ULTRAFLUAR objectives, which I just happen to have a specific 6x type (a one-off one), but there are others too.. https://www.micro-shop.zeiss.com/en/se/shop/objectives/440013-9902-000/Objective-Ultrafluar-10x-0.2# Zeiss Jena made an UV MI...
by kds315*
Mon May 25, 2020 6:04 pm
Forum: Equipment Discussions
Topic: Printing Nikkor vs Schneider Macro Varon?
Replies: 93
Views: 20129

I've been looking for a 75PN for a long time, to no avail. It was designed for 1:4/4:1 transfers, so I guess this means printing Super-8 to 35mm or vice-versa, though it has an image circle of 64mm. I would be very curious to know if anyone here has such a beast, or has ever seen or used one. Here ...
by kds315*
Sun May 17, 2020 11:33 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: White Balancing errors
Replies: 4
Views: 897

I set white balance by matching peaks in the histogram, using the upper limit sliders of a Photoshop "Levels..." adjustment layer. If I'm particularly well organized, I'll do this with a separate gray target image, then apply the same adjustment to other images shot with the same setup. But more co...
by kds315*
Wed Apr 22, 2020 1:09 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Polarized Reflected UV Photography
Replies: 9
Views: 3073

Different approach used here, delivers more intense colors...

Image
by kds315*
Sun Apr 19, 2020 11:50 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Polarized Reflected UV Photography
Replies: 9
Views: 3073

Well this is a bit on the scientific side, sheet Mica in polarized UV and visible light, different pol. angles. If interested about the colors and why, I refer to the work of Mr Michel-Levy https://en.wikipedia.org/wiki/Polarized_light_microscopy#The_Michel-Levy_chart https://a4.pbase.com/o12/08/747...
by kds315*
Sat Apr 18, 2020 9:35 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Polarized Reflected UV Photography
Replies: 9
Views: 3073

MarkSturtevant wrote:That is very interesting. I know that some flowers will use UV reflective colors to communicate with bees. Some have stripes or other patterns as 'guides' to lead bees to the center -- communication between flowers and bees that we don't normally get to see.
Thanks, yep indeed they do use this.
by kds315*
Wed Apr 15, 2020 5:27 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Polarized Reflected UV Photography
Replies: 9
Views: 3073

So today about Lewisia - Cliffmaids in reflected and polarized light

Image
by kds315*
Sun Apr 12, 2020 5:18 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Polarized Reflected UV Photography
Replies: 9
Views: 3073

Polarization is an effective tool in photography and cross-polarization is well know to allow to suppress shine and reflections from certain surfaces. Here a few tests I have made after having been able to find suitable UV polarizers using a glass vase standing on an aluminum lab jack. Visible light...
by kds315*
Sat Apr 11, 2020 1:38 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Can you make stereo pairs by rotating the subject?
Replies: 10
Views: 2314

The DNA model however works very well for me, too!
by kds315*
Fri Apr 10, 2020 2:59 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Can you make stereo pairs by rotating the subject?
Replies: 10
Views: 2314

Not outstanding at all, use stereophotomaker to adjust at least the stereo basics as my eyes hurt to see it...what were you smoking btw.?? ;-)