Search found 50 matches

by ldflan
Mon Jun 22, 2020 4:43 pm
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

Re: Nikon Diaphot TMD w/ DIC -- Help!

... My nosepiece (and I suspect all TMD nosepieces do, but maybe not) has an optic built into the "active" aperture. If only the fluorescence set-up scopes have this, then it might correct for the length increase. If all have this optic then I suspect Nikon has made this scope (with optics) to esse...
by ldflan
Sat Jun 20, 2020 11:47 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

Idflan, for multiband fluorescence, the excitation of the all the desired bands needs to be simultaneous, so you'd need multiple LEDs in very close proximity, or an optical solution for combining more widely separated lamps into a single beam, perhaps with an offset reflecting parabola fed by sever...
by ldflan
Sat Jun 20, 2020 8:09 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

Critcally was the use of a small plastic fresnel lens in front of the excitation filter, and the LED mounted at focal distance (a cm or so) in front of the cube (fresnel). No collimators or lamphouses necessary. René - Thanks. Pretty sure I can visualize what you are describing. There should be roo...
by ldflan
Sat Jun 20, 2020 8:01 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

LEDs as fluorescence excitation source is a real alternative both in expensive off the self devices and DIY systems It's what many amateurs use, including myself https://www.photomacrography.net/forum/viewtopic.php?p=201937#201937 (now improved with some modifications) The main idea is simple: put ...
by ldflan
Sat Jun 20, 2020 7:48 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

"...the easier way to spot it is the loss of parfocality between the objectives." This is very good to know! Thank you! I will look for that. "...some kind of TL compensating system [may be] present, likely a very weak negative lens or a different lens than in the original model and this could not b...
by ldflan
Wed Jun 17, 2020 2:47 pm
Forum: Equipment Exchange
Topic: WTB: Nikon CF N Plan 10x /0.30 DIC and 40x/ 0.70 DIC 160/.17
Replies: 0
Views: 447

WTB: Nikon CF N Plan 10x /0.30 DIC and 40x/ 0.70 DIC 160/.17

Thought I would see if anyone has one of either to spare... Thanks! Leonard
by ldflan
Tue Jun 16, 2020 11:51 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

I hope you put a UV barrier filter somewhere in the tube before the eyepieces!

Leonard
by ldflan
Mon Jun 15, 2020 5:20 pm
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

Hi, René - Well, higher magnifications are OK on the .55 NA DIC condenser for the Diaphot, but the results are inevitably limited and there isn't much point in using the scope as configured for that, to my way of thinking. If nothing else, using high NA objectives on the Diaphot requires super thick...
by ldflan
Mon Jun 15, 2020 10:46 am
Forum: Equipment Discussions
Topic: Nikon Diaphot TMD w/ DIC -- Help!
Replies: 25
Views: 5108

Hi Kurt. I just acquired a Diaphot with your DIC arrangement at a very reasonable price. I also have the more usual version, so I am going to do some comparisons of results. It seems pretty clear that this arrangement with separate prism and polarizer sliders below the objective turret is one that i...
by ldflan
Thu Jun 04, 2020 11:09 am
Forum: Photography Through the Microscope
Topic: Deconvolution processing of fluorescent images - Update
Replies: 4
Views: 518

Fantastic, Robert. Thanks for the link to the paper! Have you tried using any of the ImageJ deconvolution functions? I have not, but would be interested to know if anyone has and if they have a recommended workflow.

Leonard
by ldflan
Wed Jan 01, 2020 1:37 pm
Forum: Equipment Exchange
Topic: WTB: Zetopan Polarizing Stage
Replies: 0
Views: 1199

WTB: Zetopan Polarizing Stage

Prefer fully functioning stage mechanism, but will look at whatever you have if you think it can be repaired. Lots of Zetopan bits and bobs for trades, if you like.

Leonard
by ldflan
Tue Dec 03, 2019 4:38 pm
Forum: Equipment Discussions
Topic: Cooled CCD Recommendations?
Replies: 22
Views: 1691

I thought I would provide some details for future reference if someone else goes down this path or a similar one. I've fitted a QHY 163M with their 7 filter wheel and mounted it to the Nikon Diaphot's side cine port. Some pointers on doing so: 1. Select The Filter Wheel Control Pathway. Before mount...
by ldflan
Thu Nov 14, 2019 10:18 am
Forum: Equipment Discussions
Topic: Cooled CCD Recommendations?
Replies: 22
Views: 1691

Nikon has a 1x/16 TV projective eyepiece which is pretty inexpensive. A 16mm diameter circle covers a 1 inch sensor but nothing more. I have the Nikon 1x/16 TV relay lens in the cine port now, as well as a 2x and 2.5x on a shelf somewhere. One of those should do the trick, I figure. Since the .75x ...
by ldflan
Wed Nov 06, 2019 8:56 pm
Forum: Equipment Exchange
Topic: Steedman's Wax - 50g $35
Replies: 0
Views: 1026

Steedman's Wax - 50g $35

Dr. H.F. Steedman invented a polyester wax for ribbon sectioning on standard rotary microtomes in 1947. It consists of 9 parts by weight Polyethylene glycol 400 distearate melted at 60C with 1 part cetyl alcohol added. As an infiltration, embedding, and sectioning medium, Steedman's Wax has a number...
by ldflan
Sat Nov 02, 2019 10:00 am
Forum: Equipment Discussions
Topic: Cooled CCD Recommendations?
Replies: 22
Views: 1691

"This picture from the camera's manual might help:"


Indeed it does! This should be a snap.

Thanks very much!

Leonard