Search found 69 matches

by Jesse
Sun Feb 03, 2019 1:29 pm
Forum: Equipment Discussions
Topic: In search of greater depth of field
Replies: 10
Views: 2577

Lighting makes the biggest difference. Do you have some photos of your setup in use? I'm having trouble visualizing it. Also, do you use a stackshot or other automated focus rail? I'm currently using a sunwayfoto mfr-150s and while it's better than handheld for accuracy, I'm finding it very tedious...
by Jesse
Sat Feb 02, 2019 4:50 pm
Forum: Equipment Discussions
Topic: In search of greater depth of field
Replies: 10
Views: 2577

Jesse, It sounds as if you are changing the f/stop on your zoom lens. Is this really the case? If so, we need to clear up some confusion. When you mount a microscope objective on a lens such as your zoom, you are using the zoom as a "converging lens" or "tube lens." Stopping down the converging len...
by Jesse
Sat Feb 02, 2019 1:19 pm
Forum: Equipment Discussions
Topic: In search of greater depth of field
Replies: 10
Views: 2577

That ladybug image was taken with good diffused flash lighting and at a different angle. Probably generously and carefully sharpened as well. Not really comparable to your LED lighting. I was wondering about that. You think better lighting would make a huge difference? Where can I find a tutorial o...
by Jesse
Sat Feb 02, 2019 12:33 pm
Forum: Equipment Discussions
Topic: In search of greater depth of field
Replies: 10
Views: 2577

Here are some example photos I just took of a ladybug. I'm using an LED light panel for illumination. 1/160 F11 ISO 1250: https://i.imgur.com/ZOjeDY3l.jpg 1/160 F8 ISO 500: https://i.imgur.com/xgpMlT3l.jpg These are cropped to remove the majority of the image circle since this objective doesn't play...
by Jesse
Sat Feb 02, 2019 10:46 am
Forum: Equipment Discussions
Topic: In search of greater depth of field
Replies: 10
Views: 2577

In search of greater depth of field

Hello, I bought a Nikon E400 with APO objectives last year and I've been enjoying it. I spent a lot of time and money setting up a Nikon f-mount adapter for video and photography, then I spent some more time and money playing with the 4x APO objective on-camera, on the end of my 70-200mm zoom lens. ...
by Jesse
Sat Sep 29, 2018 9:05 am
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

Gah. I was wrong. The dust is on the relay lens. I thought I saw it through the binocular eyepieces, but that must be another dust speck in a similar place. Lifting up the f-mount tube enough so that I could rotate the relay lens while viewing the camera output revealed the problem.
by Jesse
Sat Sep 29, 2018 8:55 am
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

I had a moment today to work on this, so I removed the objective turret and the stage, then removed the field lens cover. Finally, I reassembled everything and rotated the field lens using a pair of tweezers. dust specks do not move. It's not the field lens.
by Jesse
Thu Sep 27, 2018 10:04 am
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

That looks frustrating. This may be an "ignorant" question, as I have little experience; have you eliminated the projective eyepiece? (rotate 90°?) If by "projective eyepiece" you mean the relay lens in the trinoc tube, then yes, I've rotated it with no result. Also the dust speck shows up in both ...
by Jesse
Wed Sep 26, 2018 5:06 pm
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

Here's the tear down video if anyone is curious. I'm linking to 7:36 where the field lens has already been removed. Dusty in there. https://youtu.be/g-W9CEiEpPA?t=7m36s
by Jesse
Wed Sep 26, 2018 12:13 pm
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

After editing the footage... it could still be the field lens. It looks to be in pretty bad shape, even after cleaning. It looked clean to the naked eye, but the camera had a much better view. I think the next thing I try will be to rotate the field lens. I'll need to strip the microscope down to do...
by Jesse
Wed Sep 26, 2018 10:07 am
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

If it is in the field lens it must focus and defocus moving the condenser knob. The method to find dirt is to rotate and focus each movable element Well, this is frustrating. The dust speck does indeed focus and defocus using the condenser knob. I had a moment, finally, to tear the microscope down ...
by Jesse
Tue Sep 11, 2018 3:11 pm
Forum: Equipment Discussions
Topic: DSLR white balance
Replies: 15
Views: 1694

Pau wrote:For video my first method will work wel for WBl.
For microscope BF you need to apply the adequate exposure compensation, if most of the frame is empty about +2EV...you'll need to perform your own tests.
Awesome. I’ll give that a try. Thanks.
by Jesse
Mon Sep 10, 2018 3:08 pm
Forum: Equipment Discussions
Topic: DSLR white balance
Replies: 15
Views: 1694

I just take a WB with no slide or with a blank part of the slide, or -more often- I shot raw an just use the eyedropper in a blank or neutral zone during conversion. Be aware that changing the halogen lamp intensity it changes colour temperature I shoot raw for stills, but I don’t have quite as muc...
by Jesse
Mon Sep 10, 2018 2:59 pm
Forum: Equipment Discussions
Topic: DSLR white balance
Replies: 15
Views: 1694

Re: DSLR white balance

What is the correct way to whitebalance and meter a brightfield scene? Depending on the body you shoot something neutral a grey card or white card I have a grey card, but I'm not sure how you'd use one under a microscope. Would help if you mentioned that it was a microscope from the start, maybe in...
by Jesse
Sun Sep 09, 2018 11:23 am
Forum: Equipment Discussions
Topic: Finding dust speck that does not move
Replies: 11
Views: 1783

Finding dust speck that does not move

Hello, When using a camera to record video with my Nikon E600 microscope via the trinocular port, I see a dust spot on my camera that does not move when I move the stage. At first I thought it must be on the camera sensor, so I cleaned the sensor using Eclipse cleaning fluid and sensor swabs. I coul...