Search found 2659 matches

by NikonUser
Fri Jun 11, 2021 8:10 am
Forum: General Discussion Forum and Community Announcements
Topic: use Bing to search PMN
Replies: 5
Views: 114

Re: use Bing to search PMN

make this sticky!
by NikonUser
Sat Jun 05, 2021 4:56 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Tongue of the bumblebee
Replies: 13
Views: 354

Re: Tongue of the bumblebee

Superb photos. Not sure about the entomological implication. The hairy tongue is for nectar Bees collect pollen on their hairy bodies and legs, they then clean themselves with legs and transfer the pollen to pollen-baskets on the hind legs. (sorry to be so pedantic, consequence of years of universit...
by NikonUser
Fri May 28, 2021 11:31 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Hand sectioning; plants
Replies: 23
Views: 4295

Re: Hand sectioning; plants

Thanks. Stained with Fast Green and Safranin-O Perhaps a better method for sectioning: https://pages.stolaf.edu/wp-content/uploads/sites/803/2017/04/Giannini_OPN_Microtome_Manual_20170415.pdf OR: The OPN Microtome: An Inexpensive, Open Source Hand-Held Mini Microtome John Giannini,* Associate Profes...
by NikonUser
Wed Apr 14, 2021 11:12 am
Forum: Equipment Exchange
Topic: Oly SPlan Apo 100x oil
Replies: 0
Views: 325

Oly SPlan Apo 100x oil

Tested today with mosquito wing tip- BHS +1.25x + 2.5x, Nikon D810, full frame image. Inserted is part of a micrometer slide taken at same time, divisions = 0.01 mm Obj Serial # 105615 I will gladly test this objective with a potential buyer's test slide (diatom?). This wing is the best I have. $600...
by NikonUser
Mon Apr 12, 2021 3:03 am
Forum: Photography Through the Microscope
Topic: When is a clam not a clam?
Replies: 5
Views: 473

Re: When is a clam not a clam?

Dave, Perhaps your shells were too worn. My FW shells were from very fresh, some alive, specimens. My text says the shells are composed of a low-magnesium calcite exoskeleton (carapace) with an outer covering, cuticle, of chitin. My guess is that it is the chitin that 'responds' to polarized light. ...
by NikonUser
Thu Apr 08, 2021 2:00 pm
Forum: Equipment Discussions
Topic: Nikon MPlan 60x ELWD NA 0.70 objective
Replies: 0
Views: 314

Nikon MPlan 60x ELWD NA 0.70 objective

I used this objective to photograph the shell of a Ostracod, last image: http://www.photomacrography.net/forum/viewtopic.php?f=27&t=43360 The image was poor. Thinking that maybe the poor result was more the nature of the subject than the quality of the lens. The shell was strongly convex. Used the s...
by NikonUser
Wed Apr 07, 2021 7:44 am
Forum: Photography Through the Microscope
Topic: Polarized midge larvae
Replies: 5
Views: 365

Re: Polarized mosquito (or midge) larvae

Excellent image; it's a Chironomid (midge)
by NikonUser
Wed Apr 07, 2021 6:33 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Seed Shrimp shell details
Replies: 3
Views: 419

Re: Seed Shrimp shell details

I photographed part of the shell with a Nikon 60x ELWD MPlan objective; NA 0.70
Rather poor image quality; not an improvement of the actual pixels from the 20x.
by NikonUser
Wed Apr 07, 2021 3:51 am
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Patterns in Nature
Replies: 4
Views: 1030

Re: Patterns in Nature

This subject:
viewtopic.php?f=27&t=43360

would fit here
by NikonUser
Tue Apr 06, 2021 2:10 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Seed Shrimp shell details
Replies: 3
Views: 419

Seed Shrimp shell details

micro_pix showed images of Ostracod shells from a marine environment. The shells appear to have many large pores: http://www.photomacrography.net/forum/viewtopic.php?f=14&t=43258&sid=2ac28e4040ae51f0d3444f0a7f41a289 I have shown images of entire animals here: http://www.photomacrography.net/forum/vi...
by NikonUser
Tue Apr 06, 2021 5:58 am
Forum: Photography Through the Microscope
Topic: Spring Sphagnum Squeeze
Replies: 10
Views: 514

Re: Spring Sphagnum Squeeze

Excellent
by NikonUser
Tue Apr 06, 2021 5:57 am
Forum: Photography Through the Microscope
Topic: Copepod carrying eggs
Replies: 2
Views: 274

Re: Copepod carrying eggs

In another post you mentioned testing your polarization setup.
A copepod such as this one of yours is a great test for polarization.
See:
https://www.microbehunter.com/microscop ... id#p101006
by NikonUser
Mon Apr 05, 2021 11:50 am
Forum: Photography Through the Microscope
Topic: When is a clam not a clam?
Replies: 5
Views: 473

When is a clam not a clam?

When it is an Ostracod! These 2-shelled Crustacea, also known as seed-shrimps , were common in a recent FW sample. They range in size, the largest in this sample is 0.60 mm long. Under polarized light the shell can show several different colours. Olympus BH2/BHS, SPlan 10x + 1.25x intermediate tube ...
by NikonUser
Sun Apr 04, 2021 2:17 pm
Forum: Photography Through the Microscope
Topic: Springtail polarized
Replies: 11
Views: 830

Re: Springtail polarized

Yes, I believe it is a full lambda plat'; labeled 530 nm.
By rotating the polarizer I can get various colour backgrounds; the setting that gives the awful purple colour also gives the best definition of the internal musculature.