Search found 386 matches

by jsp
Sun Feb 07, 2021 10:22 am
Forum: Equipment Discussions
Topic: Interchangeability of stepper motors?
Replies: 7
Views: 2319

Re: Interchangeability of stepper motors?

Hello! I'm just here to wave and say "Hello!" I built the system being referenced in the opening post, but I did it with massive amounts of help and advice from the lovely folks here on this forum and also from my husband, who is a computer scientist, so I don't necessarily know the answers that you...
by jsp
Mon Dec 24, 2018 8:06 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

I use a computer monitor to do all of my work too. I don't use the screen on the camera. I take photos via EOS Remote.

I've actually just bought my first pair of reading glasses too, as I need them to choose and position my tiny (50 cell and less) specimens.
by jsp
Sun Dec 23, 2018 2:51 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

Hello! Just popping in to post a few photos to wish you all a Merry Christmas when it comes. I got follow-on funding from my grant to buy a new computer and the photography has got radically easier now that I can stack images in 10 seconds rather than 90 minutes. Yay! Here is the obligatory tiny fer...
by jsp
Tue Jul 31, 2018 2:59 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

Hi, I just got an email from someone who wants to make a proper film about fern gametophytes. I'm interested to help and have written back, but I just wondered if I could mention here in case anyone else would like to contribute? This is the email: (Please PM if you would like the email address to r...
by jsp
Tue Jun 12, 2018 9:44 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

Hi Lou,

Thanks yes we noticed that. My collaborator was happy to get the work done fast and not worry about losing the tips, so I went with his requirements rather than take up more of his time.

I was pleased with the focused bits though. :-)
by jsp
Tue Jun 12, 2018 6:42 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

I went back today for another go and got these better images. It turns out that my collaborator didn't want close-ups of the trichomes, but rather wanted the whole leaf, so these worked out nicely with the 5x objective. That was the first time I'd used it. I used a 120 micron step size. http://www.p...
by jsp
Fri Jun 08, 2018 8:34 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

It looks a lot better cropped. Maybe this is the composition I should be aiming for and maybe I should use a higher magnification lens. I will ask the plant's owner if that would work for him. Image
by jsp
Fri Jun 08, 2018 6:34 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

This is better if I just take enough slices to get the one trichome in focus. I think if I put a black background on that it might be nice.

Image
by jsp
Fri Jun 08, 2018 6:29 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

If I am just trying to capture the shape of the trichome, do you think it would be better to go to higher magnification and just take the one trichome? That was 10x, but I now have the option of 20x and 50x.
by jsp
Fri Jun 08, 2018 6:27 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

This is the pyramid method.

Image
by jsp
Fri Jun 08, 2018 4:39 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

This is it redone with weighted average. The other one was depth map. I have one more to go but have to dash out. Thanks! This looks better already. Phew!Image
by jsp
Fri Jun 08, 2018 4:31 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

Thanks!
by jsp
Fri Jun 08, 2018 3:46 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

Hello :-) I have been to visit a collaborator at the Sainsbury Laboratory here in Cambridge and I photographed some of his Arabidopsis leaf hairs (trichomes). The trichomes are very glass-like and the compilation in Helicon focus hasn't worked very well, I think because of diffraction. I wondered if...
by jsp
Fri Mar 16, 2018 11:53 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

That's lovely :-) Thanks for showing me. :-) My setup only has three parameters that can be changed though, so it's probably safer just to stick with the arduino program and avoid introducing any bugs. This is my setup: https://github.com/BioMakers/23_Focus-stacking-system-for-gametophyte-ferns/blob...
by jsp
Sat Mar 10, 2018 8:56 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Lighting for macro photography of fern gametophytes
Replies: 600
Views: 125041

We've just changed the system to a wired camera trigger that should allow mirror lockup.

https://github.com/BioMakers/Gametophyt ... neering.md

When I say "we", I mean my husband did it. :-)