Hello!

Cross-section of the leaf Pinus heldreichii Den Ouden .
HDR-Panorama from 4 photos, stacking.
GF-Planapochromat 25x/0,65

Thank you.

ChrisR wrote:Super - what are the stains?

I used of staining: acridinrot, acriflavin, astrablau, chrysoidin.

Thank you!

Marek Mis wrote: What kind of staining have you used ? What kind of a microtome, hand one ?
Marek

I used of staining: acridinrot, acriflavin, astrablau, chrysoidin.
I used a manual cylindrical microtome (foto 9):

https://microcosmos555.blogspot.ru/p/blog-page_4.html

Cross-section of the leaf Pinus nigra.
HDR-Panorama from 4 photos
GF-Planapochromat 25x/0,65

Cross-section the rootstock Pterídium aquilínum.
HDR-panorama from 252 photos.
Objective Plan-Neofluar 16x/0,50

Thank you very much to all for your comments!

Thank you very much to all for your comments!

Hello!

Cross section of stem Physocarpus opulifolius (Rosaceae).
Plan-Neofluar 16x/0,50, HI.
Panorama of 32 Fotos.

Beautiful image. Did you stain / prepare it in any other way than slicing it? Sorry if this is a dumb question. The colours just seem supernatural. Thank you! Stained with acridin red, acriflavine and astra blue. The staining procedure was invented by Robin Wacker. https://microcosmos555.blogspot.r...

Thank you!

Thank you, Jörgen!

Pau wrote:Excellent!
few questions:
- As I read it you've imaged the very same section (and staining) for both pictures, am I right? If so these dyes produce very nice fluorescence.
- Do you cut the stem sections yourself?

Thank you!

This is one section.
I make yorself the stem sections (and staining).

zzffnn wrote:Very beautiful!

Was there a particular reason as to why you used "Planachromat VI 12,5x/0,33, water immersion"?

Thank you!
This is an experiment with water immersion

Hello!

Cross section Hordeum sp. (Poaceae).
Plan-Neofluar 16x/0,50, HI.
Panorama of 53 Fotos.

ChrisR wrote: What is "Wacker coloring"?

The coloring: Astrablau, Acridinrot, Acriflavin.
Sorry for my English...