Search found 1051 matches

by Ichthyophthirius
Sat Aug 01, 2020 3:04 am
Forum: Equipment Discussions
Topic: replacing Nikon FX-35A camera
Replies: 9
Views: 152

Re: replacing Nikon FX-35A camera

Hi, 1) The idea I described above is the following: The camera sensor is place exactly at the location of the intermediate image in/above the phototube = "direct projection". With the Nikon YT-TV adapter and Nikon 1x C-mount TV adapter A, the intermediate image should be located 17.526 mm above the ...
by Ichthyophthirius
Fri Jul 31, 2020 3:35 pm
Forum: Beginners Macro
Topic: microscope infinity optical system setup for wild bees macro photography?
Replies: 9
Views: 276

Re: microscope infinity optical system setup for wild bees macro photography?

rjlittlefield wrote:
Wed Jul 29, 2020 11:22 am
Ichthyophthirius wrote:
Wed Jul 29, 2020 1:04 am
does anyone know the NA of the MP-E at 5x?
Wide open, NA 0.149
Thanks, Rik!
by Ichthyophthirius
Fri Jul 31, 2020 3:17 pm
Forum: Equipment Discussions
Topic: Nikon's 10x finite (cf, Nikon CFI)
Replies: 3
Views: 153

Re: Nikon's 10x finite (cf, Nikon CFI)

NikonUser wrote:
Fri Jul 31, 2020 11:11 am
My objective
Hi,

Which objective was it?
by Ichthyophthirius
Fri Jul 31, 2020 2:59 pm
Forum: Equipment Discussions
Topic: replacing Nikon FX-35A camera
Replies: 9
Views: 152

Re: replacing Nikon FX-35A camera

Someone correct me if I'm wrong. It looks to me like all you need to do is to remove the entire camera part and Nikon V-T tube. https://www.lmscope.com/en/Nikon_eclipse_e400_e600_digital_Adapter_en.html Then mount a Nikon YT-TV adapter, a Nikon 1x C-mount TV adapter A and attach a C-mount to M4/3 ad...
by Ichthyophthirius
Fri Jul 31, 2020 2:40 pm
Forum: Equipment Discussions
Topic: replacing Nikon FX-35A camera
Replies: 9
Views: 152

Re: replacing Nikon FX-35A camera

I assume that any Nikon full-frame body could be used to replace the Nikon FX-35A. Please correct me if I'm wrong. If I am correct, is there any particular body I should look for (or avoid). Hi, Nikon DSLRs were unsuitable until recently because the shutters induced vibrations. Some of the newest b...
by Ichthyophthirius
Wed Jul 29, 2020 1:04 am
Forum: Beginners Macro
Topic: microscope infinity optical system setup for wild bees macro photography?
Replies: 9
Views: 276

Re: microscope infinity optical system setup for wild bees macro photography?

Hi, Just from an entomologist's, not macro, standpoint: The pictures in the paper are excellent for a scientific publication (considering the small size and the large volume of illustrations that had to be made for the paper). The stacks of the genitals and antennae with their microsculpture are out...
by Ichthyophthirius
Sat Jul 18, 2020 3:24 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Would you recommend a tripod or monopod for macro in the field?
Replies: 17
Views: 642

Re: Would you recommend a tripod or monopod for macro in the field?

Thank you all for your contributions which I keep reading with interest. The Y-joke looks interesting, never heard of that before but it makes total sense.
by Ichthyophthirius
Fri Jul 17, 2020 2:34 am
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 409

Re: staining blood with giemsa

Hi, [If you can't get the buffer, use the Evian method (link above).] I'm very sorry to hear that. As you can see, even very basic diagnostics can be difficult and are highly regulated to ensure reproducible results. I strongly advice against using any home diagnostics as a basis for treatment decis...
by Ichthyophthirius
Thu Jul 16, 2020 4:01 am
Forum: Beginners Micro
Topic: Objective projection, SwiftSW350T on to camera (Canon) sensor
Replies: 14
Views: 377

Re: Objective projection, SwiftSW350T on to camera (Canon) sensor

In that case, can you measure the dovetail dimensions (hight, min and max diameter; angle to confirm)? It looks like the Lomo/Jena dovetail but it could be anything.
by Ichthyophthirius
Thu Jul 16, 2020 3:50 am
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 409

Re: staining blood with giemsa

If you can do so, also measure the pH of your destilled water (whatever you're using) and your tap water. As you can see from the Magnacol trouble shooting instructions, the pH has a slight effect on the staining results. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC540181/
by Ichthyophthirius
Thu Jul 16, 2020 3:44 am
Forum: Beginners Micro
Topic: Meopta lens problems
Replies: 6
Views: 192

Re: Meopta lens problems

Hi, The 45:1 requires the object to be covered with a cover glass. Ideally, there should also be a mountant between the blood smear and the cover glass as well (balsam or a drop of immersion oil). The open, uncovered smear results in strong spherical aberration. https://www.olympus-lifescience.com/f...
by Ichthyophthirius
Thu Jul 16, 2020 3:32 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Would you recommend a tripod or monopod for macro in the field?
Replies: 17
Views: 642

Re: Would you recommend a tripod or monopod for macro in the field?

Hi Enrico, Hi Beatsy, Thank you for your input, that's very useful! I also use those handheld techniques when I'm walking around but it's too exhausting (especially on my eyes looking through the viewfinder) when I'm semi-stationary, like when I'm waiting for for a few minutes for insects to return ...
by Ichthyophthirius
Wed Jul 15, 2020 4:43 pm
Forum: Equipment Discussions
Topic: Questions regarding relay optics on photoeyepieces.
Replies: 1
Views: 148

Re: Questions regarding relay optics on photoeyepieces.

Yes, it works https://www.photomacrography.net/forum/viewtopic.php?f=25&t=25345 But I haven't done any systematic tests of image quality since. I was thinking about using a 0.7x camera speedbooster for the same purpose but I haven't brought myself to spend the money on this when afocal is so much ea...
by Ichthyophthirius
Wed Jul 15, 2020 3:06 pm
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 409

Re: staining blood with giemsa

I am not sure what "buffered" means in this context. It's not immediately obvious what the problem is. I do my blood smear stainings on a staining rack (slides horizontal and in the open) which doesn't use a lot of reagent if you only do a few slides. I use a Giemsa buffer for diluting the staining...
by Ichthyophthirius
Wed Jul 15, 2020 11:27 am
Forum: Beginners Micro
Topic: staining blood with giemsa
Replies: 19
Views: 409

Re: staining blood with giemsa

Hi, After washing off the stain, did you dry again? What is the colour of your dry, stained blood smear? The smear should be purple (naked eye) and the RBCs should be red under the microscope. If they remain unstained, the staining didn't work. Frequent problems are expired Giemsa dye (which dye is ...