Yep, control for temperature, oxygen in the water and all of this while not moving the subject too much. Also high depth of field suggests some stacking.
The first few divisions when you see ripples in cells are something incredible.

Just found this very cool looking video of tadpole embryo cell division.
https://www.youtube.com/watch?v=Wz4igVjNGq4
I wonder if anyone from this forum tried something similar.

Yep. If it has no means of changing focus position it will not work. But should work for microscope lenses or any other lenses on a mechanized platform.

Hi mawyatt, The idea is to have one less device to control with fewer wires around. I have a regular stacking rail with a separate controller. And I have to use one hand controller to set number of steps, number of exposures, etc. Separately I set up camera's exposure, iso, etc. And have one wire co...

Hi guys, You know what would be cool? Having Canon camera to control macro rails/microscope focusing stepper while taking images in focus stacking mode. This is not a pipe dream. Magic Lantern allows for focus stacking mode. See how it works here: https://www.youtube.com/watch?v=CcRVXuc6Yt4 While Ca...

When taking videos with many DSLRs only a third/quarter of pixels (line skipping) is used to make single frame picture with the rest discarded. So small shifts introduced by your hands or any other way can allow to retrieve information from adjacent pixels. You can try to enlarge individual frames b...

Thanks! Great pictures.
The thing looks scary. Those jaws and paws. Arghhh...
Skin is kind of gross too.

Be careful with that thing. I was once bitten by one and was (almost forcefully) hospitalized for a short period of time to receive anti-rabies vaccine. Seems like no one cares if a stray dog bites you, but if it's a bat - then you're in trouble.

You can use Iris software popular among amateur astrophotographers. It's free and supports your camera. It will give you pure raw B channel. You can get it here: http://www.astrosurf.com/buil/us/iris/iris.htm What you need is a SPLIT_CFA command (split_cfa2 for batches) http://www.astrosurf.com/buil...

I can't help returning to this topic and looking at these stunning images. Will you please share some details on how you got them. Equipment, lighting, the usual stuff.

harisA,
Objectives I used are quite old and have RMS thread.

Absolutely stunning beauty.

Thanks, zzffnn!

I used a concave slide and hydra was sitting close to its edge. Squeezed, but not too hard. 0.95x objective is a pain to work with. Working distance is next to nothing. But when it does work It gives very nice results.

Regards,
Dennis

Having tired of looking for subjects to observe during long winters I decided to set up a small aquarium with some plants and shrimps. Boy, I never looked back. The latest subject was a small 2mm long hydra. What a fascinating animal! Rich in details and very easy to observe alive. Anyway, I took th...

First one is absolutely stunning!