Well done! Quite dramatic to see the resolution improving.
BTW, are you able to make an image with a normal 100x oil immersion objective? I expect that to give a better image than such a specialized UV objective in the normal light region.
Best wishes, René
Search found 467 matches
- Thu Apr 28, 2022 2:45 am
- Forum: Photography Through the Microscope
- Topic: Diatom imaged with 313nm light
- Replies: 60
- Views: 8705
- Mon Mar 21, 2022 7:22 am
- Forum: Photography Through the Microscope
- Topic: Diatom imaged with 313nm light
- Replies: 60
- Views: 8705
Re: Diatom imaged with 313nm light
I'm not at all convinced by this report, typical tunnelvision of a couple of material scientists. But indeed Lou, instead of using a high refractive mountant to create contrast (which doesn't work in UV in any case), you can use water and still be able to generate (absorbance) contrast. As usual for...
- Fri Mar 18, 2022 7:09 am
- Forum: Photography Through the Microscope
- Topic: Snail tongues
- Replies: 23
- Views: 2612
Re: Snail tongues
Wow, amazing! How did you clean them?
René
René
- Fri Feb 25, 2022 8:37 am
- Forum: Photography Through the Microscope
- Topic: daphnia by delaminated Lomo 4.7
- Replies: 7
- Views: 1344
Re: daphnia by delaminated Lomo 4.7
Nice effect! Is this lens really delaminated, or just loss of coating?
Best wishes, René
Best wishes, René
- Tue Feb 15, 2022 4:47 am
- Forum: Photography Through the Microscope
- Topic: Warrior vs Barbarian - sponge spicule, 365 UV
- Replies: 4
- Views: 846
- Sun Dec 05, 2021 4:54 pm
- Forum: Photography Through the Microscope
- Topic: For all the desmids lovers :)
- Replies: 10
- Views: 1432
Re: For all the desmids lovers :)
Wow indeed, let me know if they come up for sale :-)
The one that shows the chloroplast structure is a nice touch.
Best wishes, René
The one that shows the chloroplast structure is a nice touch.
Best wishes, René
- Mon Nov 15, 2021 10:23 am
- Forum: Equipment Discussions
- Topic: "Mystery" antique coloured filters perhaps for microscopy?
- Replies: 9
- Views: 1572
Re: "Mystery" antique coloured filters perhaps for microscopy?
Got a box with filters pretty much like yours, marked Wratten M filters, Kodak etc, Kingsway, London. From the closure of a photographic division within an english horticultural centre. No markings on the filters, but with some written details in the box. The centre was setup in the early 1900's. Ho...
- Sun Oct 17, 2021 2:23 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Hexamethyldisilazane (HMDS) -- a drying aid
- Replies: 6
- Views: 2948
Re: Hexamethyldisilazane (HMDS) -- a drying aid
Nice collection of links, thanks Rik. The actual number for the surface tension given for HMDS is higher than I expected. Other factors might indeed be involved in explaining the working as a drying agent. HMDS is used a lot in electronics, eg https://www.biolinscientific.com/blog/wetting-in-electro...
- Thu Mar 25, 2021 1:30 pm
- Forum: Equipment Discussions
- Topic: My Vanox AH journey (was: Olympus BH-NIC DIC with Nikon CF long-barrel optics?)
- Replies: 63
- Views: 23815
Re: My Vanox AH journey (was: Olympus BH-NIC DIC with Nikon CF long-barrel optics?)
Sounds great Victor!
My most used lens is a 25x/0.6 Leitz SW with the 40xdic prism, and as you find it is not perfect. But by lowering the condenser slightly (and opening the field iris more), I find it performs satisfactory.
Regards, René
My most used lens is a 25x/0.6 Leitz SW with the 40xdic prism, and as you find it is not perfect. But by lowering the condenser slightly (and opening the field iris more), I find it performs satisfactory.
Regards, René
- Thu Jan 21, 2021 6:11 am
- Forum: Photography Through the Microscope
- Topic: horsetail impressions
- Replies: 4
- Views: 2346
- Fri Jan 15, 2021 3:56 pm
- Forum: Equipment Discussions
- Topic: fluorescence metoo
- Replies: 9
- Views: 4019
Re: fluorescence metoo
A LED lamp, but check what excitation wavelength you want to use.
Groetjes, René
Groetjes, René
- Mon Jan 04, 2021 4:23 pm
- Forum: Equipment Discussions
- Topic: Monochromatic Microscopes?
- Replies: 25
- Views: 7385
Re: Monochromatic Microscopes?
since corrections only occur in the objectives. Eh, no, the condenser plays a part too. Anyway, who's the buyer for whom you claim this benefit? The professional market has no problem with the price of the supercorrected optics available nowadays. I don't see any interest from the amateur side for ...
- Sat Jan 02, 2021 7:03 am
- Forum: Equipment Discussions
- Topic: 60x Frankenobjectiv identification
- Replies: 8
- Views: 3352
Re: 60x Frankenobjectiv identification
Actually found a link to a HPLC detector using Olympus 40x objectives in Geissler ea (2016) Microchip HPLC separations monitored simultaneously by coherent anti-Stokes Raman scattering and fluorescence detection. (can be reached via booksc.org). No mention of phase contrast or apodization however. R...
- Sat Jan 02, 2021 6:35 am
- Forum: Equipment Discussions
- Topic: 60x Frankenobjectiv identification
- Replies: 8
- Views: 3352
Re: 60x Frankenobjectiv identification
Interesting stuff. If this is coming out of a machine, it makes more sense that it is a dry lens instead of an immersion lens? I'm sure you have tried that, but you never know. Might be part of a micro UV fluorescent detector in chromatography, with a quench of the direct light. Must be a micro cel ...
- Sat Jan 02, 2021 5:07 am
- Forum: Equipment Discussions
- Topic: Leitz APO 8mm Apochromat 24X/0.65 Objective
- Replies: 2
- Views: 2174
Re: Leitz APO 8mm Apochromat 24X/0.65 Objective
Definitely a step up from a normal achromat! 37 mm parfocality though, so needs refocussing when mixed with modern 45 mm objectives. 170 vs 160 mm is not really an issue for objectives, but for the rest of the scope (particularly eyepieces). As scarodactyl says, it is no match for a more recent (pla...