Search found 116 matches
- Fri Aug 18, 2023 4:29 am
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: Calliphora vicina, proboscis and labella.
- Replies: 13
- Views: 1717
Re: Calliphora Vicina. Proboscis and labella.
Thank you, Hatchetj0e It did take a while to learn how to get the proboscis to extend and labella inflate [fortunately they are rubbery, so survive handling well but tend to elastically retract], build a suitable obervation cell in which the sides can easily be replaced because they have to be too t...
- Sat Aug 12, 2023 7:02 am
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: Calliphora vicina, proboscis and labella.
- Replies: 13
- Views: 1717
Re: Calliphora Vicina. Proboscis and labella.
Pau asked: "Any idea about the cause of the different emission colours of different parts? (I assume that all are made of chitin)" Thus is an interesting and complicated question. I don't have an answer but clues are starting to emerge from the most recent professional research. Chitin is not a homo...
- Fri Aug 11, 2023 11:19 am
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: Calliphora vicina, proboscis and labella.
- Replies: 13
- Views: 1717
Re: Calliphora vicina. proboscis and labella.
THank you for your coments. The next two images, of a labella which has not been stained or macerated, is the closest I can come to show the labella in its naturals state Prestonal_White.jpg 200417_Run_58_Not_Macerated.jpg It liife the oral surface of the labells is pressed against the substrate so ...
- Fri Aug 11, 2023 8:27 am
- Forum: Technical and Studio Photography -- Macro and Close-up
- Topic: Calliphora vicina, proboscis and labella.
- Replies: 13
- Views: 1717
Calliphora vicina, proboscis and labella.
Calliphora vicina , proboscis and labella 230410 Run_24Cd.JPG These are the labella or lobes of the oral sucker of a bluebottle blowfly: Calliphora vicina . Under the name Musca/Calliphora erythrocephala it was probably the most intensively studied of all diptera in Europe in the early twentieth ce...
- Tue Feb 12, 2019 3:07 pm
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: Not enough pixels for 20/0.75?
- Replies: 14
- Views: 8853
On Feb 8th. Rik Littlefield Wrote: On a 22.3 mm x 14.9 mm sensor, this gives 24.7 megapixels minimum. Any fewer pixels are guaranteed to not be enough; more may be required for shorter wavelength and/or losses to antialiasing filter, Bayer filter, etc. When the first HD [1920 x 1080p] video cameras ...
- Tue Feb 12, 2019 6:10 am
- Forum: Macro and Micro Technique and Technical Discussions
- Topic: 20X Mitty
- Replies: 2
- Views: 3725
Yes. I would recommend this objective, especially for its working distance. All microscope objectives produce images which have lower contrast and resolution away from the centre of the frame because of aberrations at higher field angles. With the 20x 0.42 objective the n.a. no longer increases in p...
- Thu Jan 24, 2019 2:37 pm
- Forum: Equipment Discussions
- Topic: Oil immersion condenser
- Replies: 25
- Views: 9529
Although the maximum aperture of the condenser [and so whether it is oiled or not] definitely limits the resolving power of high n.a. objectives, in a paper published in 1952 John Baker found that, in practice. the effect is less than contemporary theory predicted. http://jcs.biologists.org/content/...
- Tue Jan 22, 2019 1:59 pm
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13684
ChrisR Asked: What are the electrical requirements of a power supply for an HBO burner? Do they need a slow voltage or current start? You need a special power supply unit which will initially start the lamp with rapid 1600V DC Pulses, then when the mercury has evaporated and the arc is established t...
- Sat Jan 19, 2019 7:20 am
- Forum: Equipment Discussions
- Topic: "Special objective" for centering epi illuminators
- Replies: 6
- Views: 3408
Leica have an online video demonstrating the use of a piece of card on the stage:
https://www.leica-microsystems.com/scie ... p-housing/
Henry
https://www.leica-microsystems.com/scie ... p-housing/
Henry
- Fri Jan 18, 2019 4:50 pm
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13684
- Fri Jan 18, 2019 3:08 pm
- Forum: Equipment Discussions
- Topic: Fluorescence on Optiphot
- Replies: 34
- Views: 13684
What a fascinating discussion. ChrisR asked on Jan 16: Query: how important is Köhler illumination ? I understand that not using it wastes light and may affect contrast but is there a problem other than that as long as there's no image of the led/filament appearing anywhere? I believe that unlike di...
- Sat Jan 05, 2019 11:15 am
- Forum: Equipment Discussions
- Topic: A glut of Mitutoyo 7.5x Plan Apo Objectives ?
- Replies: 69
- Views: 22993
Back to the Mitutoyo 7.5x On 27th. December Robert OToole wrote: "This is easy to explain. These are really the same stock of only 5 or 6 lenses. I believe these people don't even own the lenses and only order the product from the supplier after you send the funds. They make a quick 40-50% profit on...
- Sat Jan 05, 2019 10:40 am
- Forum: Equipment Discussions
- Topic: Strobe & Speedlight Output Waveforms
- Replies: 8
- Views: 3252
I attach a set of curves for the MT-24EX I made when the unit was new. http://www.photomacrography.net/forum/userpix/1345_image035_1.jpg http://www.photomacrography.net/forum/userpix/1345_image037_1.jpg http://www.photomacrography.net/forum/userpix/1345_image039_1.jpg The noise on the traces is not ...
- Sat Dec 29, 2018 11:01 am
- Forum: Equipment Discussions
- Topic: "OEM Nikon 20x 0.75 VC" cover slip thickness
- Replies: 52
- Views: 15507
I bought one of the objectives marked 20x 0.75 beccause I have a Nikon E800 with a fluorescence illuminator. It has fluorescence mirror blocks including a UV block. Just need a darkfield block if I can find or improvise one. Just checked, and there is now just one left less than six hours after Lou'...
- Sat Dec 29, 2018 9:33 am
- Forum: Equipment Discussions
- Topic: "OEM Nikon 20x 0.75 VC" cover slip thickness
- Replies: 52
- Views: 15507
Thanks for the heads up. I have just bought one. The ebay listing stated that the seller started with 12 copies. There are now just 3 left. US Patent 5,920,432 to Yutaka Suenaga and others filed in 1998 and issued on 1999 includes as the "second embodiment" a design which appears to match the specif...