It is shown mounted on a Leica L 20/0.3 EF long working distance objective and used in conjunction with a 0.6NA substage condenser with wheelstop to block central rays. The resulting illumination is essentially circular oblique epi.
Lieberkühn or Lieberkuehn, preferably not Lieberkuhn
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gpmatthews
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Lieberkühn or Lieberkuehn, preferably not Lieberkuhn
This device was often mentioned in 19th century microscopy texts, but is rarely used these days. I decided to make one from plastic card lined with foil:
It is shown mounted on a Leica L 20/0.3 EF long working distance objective and used in conjunction with a 0.6NA substage condenser with wheelstop to block central rays. The resulting illumination is essentially circular oblique epi.
It is shown mounted on a Leica L 20/0.3 EF long working distance objective and used in conjunction with a 0.6NA substage condenser with wheelstop to block central rays. The resulting illumination is essentially circular oblique epi.
Graham
Though we lean upon the same balustrade, the colours of the mountain are different.
Though we lean upon the same balustrade, the colours of the mountain are different.
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gpmatthews
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This is the classic form of wheelstop made out of metal, but they can quickly be made by sticking a patch of electrician's insulating tape in the centre of a clear plastic disc. It sits in the substage filter holder at the lower focal plane of the substage condenser. Correct size is by experimentation.
Graham
Though we lean upon the same balustrade, the colours of the mountain are different.
Though we lean upon the same balustrade, the colours of the mountain are different.
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Craig Gerard
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Thanks for sharing this Graham.
I attached one of my objectives (Nikon 10x M Plan MI) to an Olympus CH2 microscope and noticed a similar effect with brightfield.
The objective has a chrome area surrounding the front of the objective that reflects some light back onto the subject (unintentional type of Lieberkühn); but I took note of it because the result was pleasing and applicable in some instances.
The Olympus objectives that came with the CH2 don't have such a feature and their WD is shallow.
Craig
*edit: oops, typo
I attached one of my objectives (Nikon 10x M Plan MI) to an Olympus CH2 microscope and noticed a similar effect with brightfield.
The objective has a chrome area surrounding the front of the objective that reflects some light back onto the subject (unintentional type of Lieberkühn); but I took note of it because the result was pleasing and applicable in some instances.
The Olympus objectives that came with the CH2 don't have such a feature and their WD is shallow.
Craig
*edit: oops, typo
Last edited by Craig Gerard on Sun Jun 13, 2010 4:07 am, edited 1 time in total.
To use a classic quote from 'Antz' - "I almost know exactly what I'm doing!"
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Planapo
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Interesting diy method! Thanks!
That even I should be able to accomplish.
The taped reflector that is, alas not this nice metal wheel stop 
Graham, I took the liberty of expanding your title (from "Lieberkuhn" to "Lieberkühn or Lieberkuehn, preferably not Lieberkuhn") so that this posting on Lieberkühn reflector illumination can be retrieved more easily in the future. I hope you don't mind, but if you do, please let me know.
--Betty
That even I should be able to accomplish.
The taped reflector that is, alas not this nice metal wheel stop Graham, I took the liberty of expanding your title (from "Lieberkuhn" to "Lieberkühn or Lieberkuehn, preferably not Lieberkuhn") so that this posting on Lieberkühn reflector illumination can be retrieved more easily in the future. I hope you don't mind, but if you do, please let me know.
--Betty
Last edited by Planapo on Sun Jun 13, 2010 3:25 am, edited 1 time in total.
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gpmatthews
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No problem, Betty - I used "Lieberkuehn" in my posting of the aphid picture at http://www.photomacrography.net/forum/v ... php?t=9971 using this device - an oversight misspelling the title here
Graham
Though we lean upon the same balustrade, the colours of the mountain are different.
Though we lean upon the same balustrade, the colours of the mountain are different.
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Planapo
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- Location: Germany, in the United States of Europe
Thanks for the link, Graham! I had not seen your aphid photograph before. And it's good that we now have this posting in the technique section on your reflector linked to a photo shot with this reflector showing how really well it performs.
And besides I hope that I am not regarded as nit-picky concerning the spelling of the name. It's just that poor Mr. Lieberkühn gets mutilated so often.
--Betty
And besides I hope that I am not regarded as nit-picky concerning the spelling of the name. It's just that poor Mr. Lieberkühn gets mutilated so often.
--Betty