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Tonikon

Joined: 30 Oct 2008
Posts: 52
Location: Italy

 Posted: Thu Sep 03, 2009 1:02 am    Post subject: Tube lenght, magnification and working distance Hi everyone, the "official" formula to calculate the focal lenght of microscope objectives is: Focal lenght = (Tube lenght / M + 1). But this formula is good only for a "thin lens" and is scarcely usable for a real lens. Moreover (and above all), two objectives with the same magnification (for example Olympus PlanApo 10x and Olympus MPlan 10x, both 160mm), but with very different working distances (respectively 0.16mm and 7.10 mm) can't have the same focal lenght. So, I ask myself if there really is a formula that uses tube lenght, magnification an working distance to give the correct focal lenght. Ciao Toni
rjlittlefield

Joined: 01 Aug 2006
Posts: 19895
Location: Richland, Washington State, USA

Posted: Thu Sep 03, 2009 8:25 am    Post subject: Re: Tube lenght, magnification and working distance

 Tonikon wrote: So, I ask myself if there really is a formula that uses tube lenght, magnification an working distance to give the correct focal lenght.

No, there is not. Two lenses with the same magnifications and working distances can have different focal lengths. The focal length can be computed from magnification, distances, and positions of the two "principal planes" of the lens. But there is no way to know where those planes are without doing some other measurements.

However, you can determine the focal length by measuring magnification at two different extensions and working carefully through the algebra that relates total extension to focal length and magnification.

If I have done the algebra correctly, an intermediate stage is just

FL = (e1-e2)/(m1-m2)

where FL is focal length, e1 and e2 are different extensions, and m1 and m2 are the corresponding magnifications. Of course you don't know what the total extensions are, but you do know the difference between them. So then the formula reduces to

FL = (difference of extensions) / (difference of magnifications)

I have not experimentally checked this result and I don't have a reference for it. Please let me know if it does or doesn't work.

--Rik
Tonikon

Joined: 30 Oct 2008
Posts: 52
Location: Italy

 Posted: Thu Sep 03, 2009 12:59 pm    Post subject: Thank you very much, Rik You are really right, we have to use two magnifications at two different extensions...simply ingenious! I have calculated some different magnifications with 165mm and 193mm...only 28mm of difference in tube lenght (because I have already available these values) but tomorrow I'll make other measurations with more difference in tube lenght. Applying this formula with some objectives (with known focal lenght) I have obtained: Zeiss Luminar 63mm f/4.5 = Focal lenght resulting: 56mm Canon 35mm f/2.8 Photomicro = Focal lenght resulting: 31mm Minolta 12.5mm f/2.8 = Focal lenght resulting: 9mm These results are in good accord with nominal values, but probably using more difference in tube lenght I'll obtain more correct values). Using the same formula, it results that my Nikon U10 0.22 (a 10x for metallurgical microscope) is a 20mm and my Olympus MPlan 1.3x 0.03 is a 52mm. Thank you for your precious suggestion. Ciao Toni
Tonikon

Joined: 30 Oct 2008
Posts: 52
Location: Italy

 Posted: Fri Sep 04, 2009 2:20 pm    Post subject: Hi everyone, I have just tried the formula suggested by Rik using a deeper tube lenght difference (56mm) and results was exciting! Olympus MPlan 1.3x 0.03 = Focal lenght calculated: 70mm Luminar 63mm f/4.5 = Focal lenght calculated: 64,5mm Canon 35mm f/2.8 Photomicro = Focal lenght calculated: 34,5mm Nikon U10 0.22 = Focal lenght calculated: 21.4 mm Leitz 10x 0.25 170mm = Focal lenght calculated: 15.7mm Luminar 63 and Canon 35 was used as "controller" lenses and the focal lenght calculated was very very similar to nominal focal lenght. This formula (thank you very much, Rik) is perfect to calculate experimentally the focal lenght of microscope objectives! Nikon U10 and Leitz 10x (the first one has a very long WD, about 15mm) have very different focal lenght , how it's reasonable to expect! Ciao Toni
rjlittlefield

Joined: 01 Aug 2006
Posts: 19895
Location: Richland, Washington State, USA

 Posted: Fri Sep 04, 2009 2:59 pm    Post subject: Thanks for the feedback, Toni. It's nice to know that the formula actually works. I never trust the math by itself! --Rik
mgoodm3

Joined: 08 Sep 2008
Posts: 273
Location: Southern OR

 Posted: Fri Sep 04, 2009 8:48 pm    Post subject: Another way to get reasonably close to the focal length is to measure the distance from the detector to the object you are imaging (may need to measure from the lens mount and add the flage distance fo rthe camera) then measure the magnification at the detector with a ruler or stage micrometer. fl = distance measured/( m + 1/m + 2) this neglects the distance between the principal planes. On a side note you could find the distance between the principal planes by combining the methods. Calulate the focal length by Rik's method and plug it into the fomula with the same magnificatiob and calculate the expected distance, subtract it form the real distance and you have the distance between the principal planes... theoretically, if you have accurate enough measurements
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