www.photomacrography.net :: View topic - Eye of fruit fly
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Eye of fruit fly

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 17611
Location: Richland, Washington State, USA

PostPosted: Thu May 14, 2009 11:22 pm    Post subject: Eye of fruit fly Reply with quote



I thought I'd try an eye with setae, just for comparison.

Compare this with the naked fly's eye at http://www.photomacrography.net/forum/viewtopic.php?t=7373.

This is a fruit fly, about 1/2 frame.

Canon 300D camera, Olympus CH microscope base, Nikon CF M-Plan 20X NA 0.40 ELWD objective at 21X, pingpong ball diffuser with dual fiber halogen illumination, 0.5 second at ISO 100, 106-frame stack at 4 microns focus step, Zerene Stacker PMax, no retouching.

It's interesting to notice, in this picture, the ommatidia at lower left. They appear to be very complicated. But in fact, they are just smooth shiny bumps --- it is their environment that is very complicated! In each ommatidium, the dark disk is a reflection of the microscope objective, while the lighter ring is a reflection of the illuminated pingpong ball. The radial rays are simply reflections of the surrounding setae, of which there is one at almost every junction between ommatidia. One of the advantages of electron microscopy, such as shown at http://commons.wikimedia.org/wiki/File:Drosophilidae_compound_eye_.jpg, is that there are no confusing reflections.

--Rik

Edit: to provide a new link to a scanning electron image, since the previous one disappeared.


Last edited by rjlittlefield on Wed May 26, 2010 1:45 pm; edited 1 time in total
Back to top
View user's profile Send private message Visit poster's website
NikonUser



Joined: 04 Sep 2008
Posts: 2521
Location: southern New Brunswick, Canada

PostPosted: Fri May 15, 2009 1:21 pm    Post subject: Reply with quote

That's amazing detail. I reckon such detailed, high mag, high resolution, images of parts of bugs would be effective in separating sibling species. I can't imagine that sibling species that defy separation based on viewing under a low power stereo scope would not show reliable differences with images such as yours. The trick, of course, is to know what bits to look at.
Jeff Freman and I used the arrangement of ommatidia in the eyes of male greenhead flies (Diptera, Tabanidae, Tabanus nigrovittatus complex) to unequivocally separate/identify 2 of these species.
_________________
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives
Back to top
View user's profile Send private message
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 17611
Location: Richland, Washington State, USA

PostPosted: Fri May 15, 2009 4:17 pm    Post subject: Reply with quote

NikonUser wrote:
The trick, of course, is to know what bits to look at.

Very true. I can write software and arrange lenses, but I have no idea what images would be useful to an entomologist.

So I figure the best thing I can do for you guys is to develop technology and illustrate a few possibilities.

Figuring out what to do with the technology . . . that ball is in your court. Smile

--Rik
Back to top
View user's profile Send private message Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 17611
Location: Richland, Washington State, USA

PostPosted: Wed May 26, 2010 1:51 pm    Post subject: Reply with quote

Here is a stereo rendition of the eye. This is to complement recent discussion of rendering issues regarding an eye shot earlier at lower magnification, HERE.



Zerene Stacker PMax, synthetic stereo at +-2.5%.

--Rik
Back to top
View user's profile Send private message Visit poster's website
svalley



Joined: 03 Dec 2006
Posts: 259
Location: Albany, Oregon

PostPosted: Fri May 28, 2010 2:40 pm    Post subject: Reply with quote

Interesting shots, Rik.

I have been shooting a few Fruit Flies lately myself.We are dealing with a nasty invasive pest, Drosophila suzukii and trying to teach people to be able to identify it compared to numerous other small flies.

Steve
_________________
"You can't build a time machine without weird optics"
Steve Valley - Albany, Oregon
Back to top
View user's profile Send private message Send e-mail
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group