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No life in a drop of water!

 
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sat Oct 14, 2006 11:40 am    Post subject: No life in a drop of water! Reply with quote

Some water has collected in a large tub outside and it has mosquito larvae in it,so i thought I'd have a dip and find some nice algae or rotifers.
But there was absolutely nothing moving in my sample, and precious little organic matter(I guses I should have scraped the bottom where some dried grass has fallen in)

All i could find that looked remotely organic was this collection of what looks like protoplasm. I was hoiping I'd found an amoeba but it never moved!



I shifted the camera slightly to partially obscure the image as this showed some off acxis detail-gave a slightly 3D look:

http://img.photobucket.com/albums/v11/Dawnrider/Nature/Through%20the%20Microscope/protoplasm02.jpg
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Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope
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gpmatthews



Joined: 03 Aug 2006
Posts: 1028
Location: Horsham, W. Sussex, UK

PostPosted: Sat Oct 14, 2006 12:54 pm    Post subject: Reply with quote

Try some scrapings from the sides and also some sediment or scrapings from the bottom. Another good source of animalcules in the absence of a pond is a bird-bath. Usually lots of bdelloid rotifers, especially Philodina

Also, just soak some organic matter, such as dead leaves, a couple of grains of rice, some moss etc. in a little water for a few days - you should get lots of creatures. It would be best to use rainwater, such as some from your disappointing sample.

You could try looking at the mosquito larvae - if they wriggle too much, give them a drop of sore throat anaesthetic spray - a little lidocaine paralyses them a treat.
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Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sat Oct 14, 2006 1:09 pm    Post subject: Reply with quote

I could do with some Lidocaine for my sore throat!

Philodina! I remember drawing that thru my scope years ago!


Hey I wonder what I'm likely to find in my compost bin?
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Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7058
Location: lat=35.4005&lon=-81.9841

PostPosted: Sat Oct 14, 2006 1:11 pm    Post subject: Reply with quote

I agree with Graham. About a pint of water from the tub in a glass jar and then add some dried crushed leaves to it. Shouldn't be more than a couple of days and you should have a jar full of beasties. Very Happy
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