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Greetings from Cologne

 
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Jamie V.



Joined: 07 Jan 2009
Posts: 5
Location: Cologne, Germany

PostPosted: Wed Jan 07, 2009 10:47 am    Post subject: Greetings from Cologne Reply with quote

I just joined, today. As I am currently starting a project in cytometry, whereby I will be fotographing plant chromosomes and have absolutely no experience in this area, I thought I may find some useful infos here.
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Jamie

Cologne
Germany

waking escapes the dream
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7048
Location: lat=35.4005&lon=-81.9841

PostPosted: Wed Jan 07, 2009 7:07 pm    Post subject: Reply with quote

Greetings Jamie Very Happy There are a great many talented microscopist on this forum and I would pretty much bet you will find an abundance of help. Sounds like an interesting project that you are embarking upon. Anyway there Jamie, welcome aboard. I hope that you enjoy the forums. A lot and I mean a "lot" of great talent here. Very Happy
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Harold Gough



Joined: 09 Mar 2008
Posts: 5787
Location: Reading, Berkshire, England

PostPosted: Thu Jan 08, 2009 12:03 am    Post subject: Reply with quote

Jamie,

I'm not sure if this helps but I believe that fruit flies Drosophila have giant chromosomes and could be an entry point for developing your technique.

Harold
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Jamie V.



Joined: 07 Jan 2009
Posts: 5
Location: Cologne, Germany

PostPosted: Thu Jan 08, 2009 5:53 am    Post subject: Reply with quote

Thanks for the welcome!

Harold,

I've already managed to view onion chromosomes with little trouble at 100X mag. With my planned work in Iris and Hemerocallis, I expect it is more the preperation of the slides that is going to prove the bear. I will be using the squash technique with aceto-orecin, aceto-carmin and Giemsa banding stains. If I can master these, next year I want to go on to FISH, but the microscope will be a major purchase.

And all this because I'm a curious person who just wants to know.....
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Jamie

Cologne
Germany

waking escapes the dream
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