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Unidentified ciliate

 
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gpmatthews



Joined: 03 Aug 2006
Posts: 1033
Location: Horsham, W. Sussex, UK

PostPosted: Sun Sep 17, 2006 3:38 pm    Post subject: Unidentified ciliate Reply with quote




Zeiss Standard GFL Microscope
Ocular: Watson x8 Compensating
Objective: Leitz 40/0.7 ICT Fluotar
Substage: Leitz ICT
DIC
Sample from garden pond
Camera: Canon Powershot S50
Flash

Here are a selection of pictures of this ciliate. It was rather scoop shaped, or possibly a little like a grain of wheat, with a central groove, at one end of which was a large feature, probably the mouth. You can see its 3D shape in the bottom RH picture of the 1st set, and on the bottom pictures of the 2nd set. The bottom left picture of the 1st set shows the central depression in plan view. Its mode of swimming was very smooth and not too fast, with the occasional pirhouette. It appeared to be semi-rigid, deforming fairly easily, but always returning to the same shape. The surface was covered with rows of kineties.

Any ideas on identity?
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Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7076
Location: lat=35.4005&lon=-81.9841

PostPosted: Sun Sep 17, 2006 4:20 pm    Post subject: Reply with quote

I've seen this thing before too. I have looked all over but cannot find anything that closely resembles this ciliate. Confused You have some good shots of it Graham, I was hoping the Protist Information Server would turn up something but it didn't, maybe I was looking in the wrong places. Think
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Thomas Ashcraft



Joined: 07 Aug 2006
Posts: 133

PostPosted: Sun Sep 17, 2006 4:34 pm    Post subject: Re: Unidentified ciliate Reply with quote

Just guessing, but maybe a variety of Lembadion?

http://starcentral.mbl.edu/microscope/portal.php?pagetitle=taxonfactsheet&type=organism&taxon=Lembadion

Tom
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7076
Location: lat=35.4005&lon=-81.9841

PostPosted: Sun Sep 17, 2006 4:38 pm    Post subject: Reply with quote

Could be Tom, but there are numerous cell inclusions not normally found in Lembadion or I would think so. There seems to be a postoral groove present in this organism that leads me to assume, maybe, that it is Frontonia leucas but still there are some organells missing there too. So I am still not sure. However you could be correct in your identification also. Very Happy
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Thomas Ashcraft



Joined: 07 Aug 2006
Posts: 133

PostPosted: Sun Sep 17, 2006 4:54 pm    Post subject: Reply with quote

Ken Ramos wrote:
Could be Tom, but there are numerous cell inclusions not normally found in Lembadion or I would think so. There seems to be a postoral groove present in this organism that leads me to assume, maybe, that it is Frontonia leucas but still there are some organells missing there too. So I am still not sure. However you could be correct in your identification also. Very Happy


Ken, Graham,

Here's an image from the protist website of Lembadion.

http://protist.i.hosei.ac.jp/PDB/Images/Ciliophora/Lembadion/Lembadion.jpg

Maybe Graham's specimen could have a larger scoop for it to be Lembadion.

Not sure.

Tom
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Thomas Ashcraft



Joined: 07 Aug 2006
Posts: 133

PostPosted: Sun Sep 17, 2006 5:34 pm    Post subject: Reply with quote

I made a hasty webpage of a possible Lembadion ( not positive ) with a 20 second video shot at 1000x magnification bight field. I'm not sure if this is similar to Graham's specimens but at 16 seconds into the movie the protozoan does a couple of possible "pirhouettes."

http://www.heliotown.com/Possible_Lembadion.html

Tom
(Hoping to get better at identifying, using mostly D. J. Patterson's Free-Living Freshwater Protozoa and also Jahn's How to Know the Protozoa. )
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Sun Sep 17, 2006 10:37 pm    Post subject: Reply with quote

Don't know Graham.

Thomas... I am pretty certain yours (as referenced in you reply) is indeed a Lembadian.
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Wim van Egmond



Joined: 15 Aug 2006
Posts: 826
Location: Berkel en Rodenrijs, the Netherlands

PostPosted: Mon Sep 18, 2006 5:31 am    Post subject: Reply with quote

I am not sure if Graham's ciliate is a Lembadion. Lembadion has a large undulating membrane. I'll post one.

Wim
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gpmatthews



Joined: 03 Aug 2006
Posts: 1033
Location: Horsham, W. Sussex, UK

PostPosted: Mon Sep 18, 2006 11:57 am    Post subject: Reply with quote

I'm not sure about Lembadion- my own photos show it at around 50 microns total length making it rather small. It also has long caudal cilia, which this latest specimen lacks. Just have to keep looking...
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Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
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bernhardinho



Joined: 13 Aug 2006
Posts: 560
Location: Germany

PostPosted: Mon Sep 18, 2006 1:04 pm    Post subject: Reply with quote

Hi friends,

well as far as size is concerned, I read in my books that L.lucens is small (50-70µm). But L.bullinum or L.magnum are larger (100-200µm).

Best wishes

Bernhard
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