Paramecium bursara
Paramecium bursara
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- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
Paramecium bursara
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
Paramecium bursara
It was a 40X objective, focus stacked series. I have been experimenting with illumination effects at high magnifications - mostly off-set illumination, which results in more three-dimensional relief at the cost of lesser resolution. I have a certain home-made spider darkfield stop that when used a 20X objective provides amazing results. When that same stop is used at 40X or higher, by "feathering" the edge of the spider stop can provide an informative cross-illumination at higher magnifications.
Mike
Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
It's surprising how much more you can see using oblique-type illumination, though I always find it difficult to photograph. You've captured it well here. I struggle to do oblique on my Zeiss inverted (not enough suitable adjustments in the illumination train) but I (re)discovered oblique on my Nikon Labophot. Just using a hand to shade some of the light coming in from underneath works a treat - and it's quick and easy to move the hand around for different effects.
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- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
Oblique lighting
Thanks, everyone. I started experimenting feathering a spider (darkfield) stop at high magnification after finally figuring out high magnification (60X objectives) Rheinberg illumination. The result is a form of the "Limb Effect" which appears to impart a cross-lighting effect.
Mike
Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA