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Autumn pond organism & drawing effect with wrong phase r

 
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Robert Berdan



Joined: 18 Oct 2017
Posts: 73
Location: Calgary

PostPosted: Tue Oct 09, 2018 9:24 am    Post subject: Autumn pond organism & drawing effect with wrong phase r Reply with quote

We have an early winter in Calgary with over 20 cm of snow so far. I returned recently from the arctic so the cold weather doesn't seem so bad, however pond life is undergoing a radical change. I had so much fun over the summer taking pictures of pond life that I am setting up a small aquarium to house invertebrates over the winter. This way I can have material to photograph throughout the winter. I will still explore what is living in ponds below the ice.

Below are a few recent photomicrographs I would like to share - I am working on a couple of articles for my web site where I will post more pictures once I can identify many of the new organisms I have found.

I use a Zeiss Axioscope with a Nikon D500 attached and I was playing with Phase contrast and found if I used a 20X objective with the Phase I ring (for 10X) I got a neat effect that almost looks like a drawing that might be done in Photoshop - only it wasn't - see Brachionus rotifer photos. I have not seen this effect before.



First 3 photos rotifer freshwater Brachionus sp
Top photo - rotifer with wrong phase ring
Second photo - rotifer with Darkfield microscopy
Third photo - rotifer with phase contrast (positive)
Fourth photo - freshwater snail embryo DIC microscopy - 5X objective
Fifth photo - Desmid Spinocosmarium sp DIC microscooy - 63X objective
Sixth photo - Pandorina 40X objective DIC - 32 biflagellate cells
Seventh Low mag DIC 2.5X objective, Insect larvae - water bug hemiptera Agrion damselflie larva









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Harald



Joined: 13 May 2011
Posts: 492
Location: Steinberg, Norway

PostPosted: Tue Oct 09, 2018 1:07 pm    Post subject: Reply with quote

Hi there,
This is great. I excited to follow you through the winter.
There is no snow in the part of Norway where I live so far.

You have given me some inspiration here and thanks for sharing Very Happy
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Harald

Lier Fotoklubb / NSFF
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Marek Mis



Joined: 10 Jul 2010
Posts: 2519
Location: Suwalki, Poland

PostPosted: Tue Oct 09, 2018 9:09 pm    Post subject: Reply with quote

The surprising effect in the first photo indeed, Robert.
Sometimes incorrect using of any technique of the illumination gives surprising and interesting results.

Marek
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Jacek



Joined: 02 Oct 2011
Posts: 5120
Location: Poland

PostPosted: Wed Oct 10, 2018 3:19 am    Post subject: Reply with quote

Very nice
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