Fern Sporangia, opened up & empty >more fluorescence

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

WalterD
Posts: 616
Joined: Mon Jul 06, 2015 12:01 pm
Location: Rotterdam, the Netherlands
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Post by WalterD »

Hi Frank, Pierre and Chris, many thanks for your positive feedback.

@ Chris:
Your Beautiful front-page image - what wavelengths is that working with?
This was done with the YOC-6-3 excitation filter (3mm thick) combined with the blue filter block. The YOC-6-3 works with ultraviolet excitation, see below curve.

Image

According Crystaltechno (a producer of similar filters) the curve should look like this:

Image


The barrier filter in the filter block itself (with Lomo always separate from the excitation filters) has a transmission bandwith from 420 to 500 nm. This is basically blue light, while the actual picture shows more than that.
...What do you think?

Image


Below photo was done with the same combination. The colours are not completely consistent with those in previous homepage image. This may be because of -besides different post-processing- it's a different fern and the relay collector lens was upgraded by an additional concave lens in the front.
Image

Jacek
Posts: 5357
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

SUPER !

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