Fern sori
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Fern sori
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
Autofluorescence in fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
IMG_0195-Edit.jpg by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
Thanks. FLUO-C6.1 means autofluorescence, and I used my cube labelled C6.1 which refers to specific dichroic mirror, bandpass filter and excitation filter.Lou Jost wrote:Wow, fantastic. Sharp and with a nice wide range of colors. Not knowing what some of the terms mean, can you explain in words what "FLUO-C6.1, HF B+C" means?
HF is Helicon Focus for depth of field stacking, B+C means I blended a method B and a method C stacking into one image.
Excitation Filter: 352 – 402 nm single band bandpass filterLou Jost wrote:Thanks, I should have recognized the Helicon abbreviations. The details of the filters and excitation source would be very interesting to know. Maybe you have referred to it in another post somewhere that you could refer us to?
Dichroic Mirror: 414nm
Emission Filter: 437nm long pass edge filter
Excellent images, Waldo
This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?
This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?
Pau
Thanks Pau. I agree - photobleaching is definitely a concern here. My ISO was 400. However I had a 1.6 second exposure time per frame. My X-Cite 120 was set to its lowest level like all my photos. I definitely do engage ND filters - but I never record which ones. I have a 0.25 and 0.6. Sometimes I'll use both, but this image lets me believe I only engaged the 0.6 or the 0.25.Pau wrote:Excellent images, Waldo
This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?
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