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Fern sori
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pwnell



Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada

PostPosted: Tue Apr 10, 2018 3:03 pm    Post subject: Fern sori Reply with quote


Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C


Autofluorescence in fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C


IMG_0195-Edit.jpg by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C


Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C


Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C


Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
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vasselle



Joined: 05 Jan 2014
Posts: 1497
Location: France

PostPosted: Tue Apr 10, 2018 3:33 pm    Post subject: Reply with quote

Bonjour
Superbes images
Cordialement seb
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Jacek



Joined: 02 Oct 2011
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Location: Poland

PostPosted: Wed Apr 11, 2018 4:34 am    Post subject: Reply with quote

Super.
The capsules did not break, throwing out pollen?
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Lou Jost



Joined: 04 Sep 2015
Posts: 2859
Location: Ecuador

PostPosted: Wed Apr 11, 2018 4:57 am    Post subject: Reply with quote

Wow, fantastic. Sharp and with a nice wide range of colors. Not knowing what some of the terms mean, can you explain in words what "FLUO-C6.1, HF B+C" means?
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JH



Joined: 09 Mar 2013
Posts: 1175
Location: Vallentuna, Stockholm, Sweden

PostPosted: Wed Apr 11, 2018 8:30 am    Post subject: Reply with quote

Impressive work.
Best regards
Jörgen Hellberg
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pwnell



Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada

PostPosted: Wed Apr 11, 2018 9:12 am    Post subject: Reply with quote

Lou Jost wrote:
Wow, fantastic. Sharp and with a nice wide range of colors. Not knowing what some of the terms mean, can you explain in words what "FLUO-C6.1, HF B+C" means?

Thanks. FLUO-C6.1 means autofluorescence, and I used my cube labelled C6.1 which refers to specific dichroic mirror, bandpass filter and excitation filter.

HF is Helicon Focus for depth of field stacking, B+C means I blended a method B and a method C stacking into one image.
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Lou Jost



Joined: 04 Sep 2015
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Location: Ecuador

PostPosted: Wed Apr 11, 2018 9:25 am    Post subject: Reply with quote

Thanks, I should have recognized the Helicon abbreviations. The details of the filters and excitation source would be very interesting to know. Maybe you have referred to it in another post somewhere that you could refer us to?
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pwnell



Joined: 18 Dec 2009
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Location: Tsawwassen, Canada

PostPosted: Wed Apr 11, 2018 4:21 pm    Post subject: Reply with quote

Lou Jost wrote:
Thanks, I should have recognized the Helicon abbreviations. The details of the filters and excitation source would be very interesting to know. Maybe you have referred to it in another post somewhere that you could refer us to?


Excitation Filter: 352 – 402 nm single band bandpass filter 
Dichroic Mirror: 414nm 
Emission Filter: 437nm long pass edge filter
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Lou Jost



Joined: 04 Sep 2015
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PostPosted: Wed Apr 11, 2018 8:27 pm    Post subject: Reply with quote

Thanks very much for those details!
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Pau
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Joined: 20 Jan 2010
Posts: 4528
Location: Valencia, Spain

PostPosted: Thu Apr 12, 2018 12:11 am    Post subject: Reply with quote

Excellent images, Waldo

This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?
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micro_pix



Joined: 11 May 2012
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PostPosted: Thu Apr 12, 2018 3:07 am    Post subject: Reply with quote

Stunning images.

David
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pwnell



Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada

PostPosted: Thu Apr 12, 2018 8:48 am    Post subject: Reply with quote

Pau wrote:
Excellent images, Waldo

This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?


Thanks Pau. I agree - photobleaching is definitely a concern here. My ISO was 400. However I had a 1.6 second exposure time per frame. My X-Cite 120 was set to its lowest level like all my photos. I definitely do engage ND filters - but I never record which ones. I have a 0.25 and 0.6. Sometimes I'll use both, but this image lets me believe I only engaged the 0.6 or the 0.25.
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Pau
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Joined: 20 Jan 2010
Posts: 4528
Location: Valencia, Spain

PostPosted: Thu Apr 12, 2018 9:03 am    Post subject: Reply with quote

Thank you very much, this is relevant info, I usually shot at ISO 100 or 200 and get exposures about 1s, so I'll need to try lowering the excitation by means of ND
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Roel Wijtmans



Joined: 23 Nov 2016
Posts: 55
Location: Sweden

PostPosted: Thu Apr 12, 2018 9:14 am    Post subject: Reply with quote

Stunning photos!
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Sumguy01



Joined: 28 Jan 2013
Posts: 1187
Location: Ketchikan Alaska USA

PostPosted: Sun Apr 15, 2018 10:42 am    Post subject: Reply with quote

Applause Amazing set.
Thanks for sharing.
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