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pwnell
Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada
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 Posted: Tue Apr 10, 2018 3:03 pm Post subject: Fern sori |
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Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
Autofluorescence in fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
IMG_0195-Edit.jpg by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 4x, FLUO-C6.1, HF B+C
Fern sori by Waldo Nell, on Flickr
Fern sori, butchard gardens, 10x, FLUO-C6.1, HF B+C |
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vasselle
Joined: 05 Jan 2014
Posts: 1497
Location: France
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| Posted: Tue Apr 10, 2018 3:33 pm Post subject: |
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Bonjour
Superbes images
Cordialement seb
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Microscope Leitz Laborlux K
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Jacek
Joined: 02 Oct 2011
Posts: 5118
Location: Poland
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Lou Jost
Joined: 04 Sep 2015
Posts: 2859
Location: Ecuador
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| Posted: Wed Apr 11, 2018 4:57 am Post subject: |
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Wow, fantastic. Sharp and with a nice wide range of colors. Not knowing what some of the terms mean, can you explain in words what "FLUO-C6.1, HF B+C" means?
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Lou Jost
www.ecomingafoundation.wordpress.com
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JH
Joined: 09 Mar 2013
Posts: 1175
Location: Vallentuna, Stockholm, Sweden
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| Posted: Wed Apr 11, 2018 8:30 am Post subject: |
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Impressive work.
Best regards
Jörgen Hellberg
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Jörgen Hellberg, my webbsite www.hphoto.se |
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pwnell
Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada
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| Posted: Wed Apr 11, 2018 9:12 am Post subject: |
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| Lou Jost wrote: | | Wow, fantastic. Sharp and with a nice wide range of colors. Not knowing what some of the terms mean, can you explain in words what "FLUO-C6.1, HF B+C" means? |
Thanks. FLUO-C6.1 means autofluorescence, and I used my cube labelled C6.1 which refers to specific dichroic mirror, bandpass filter and excitation filter.
HF is Helicon Focus for depth of field stacking, B+C means I blended a method B and a method C stacking into one image. |
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Lou Jost
Joined: 04 Sep 2015
Posts: 2859
Location: Ecuador
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| Posted: Wed Apr 11, 2018 9:25 am Post subject: |
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Thanks, I should have recognized the Helicon abbreviations. The details of the filters and excitation source would be very interesting to know. Maybe you have referred to it in another post somewhere that you could refer us to?
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Lou Jost
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pwnell
Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada
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| Posted: Wed Apr 11, 2018 4:21 pm Post subject: |
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| Lou Jost wrote: | | Thanks, I should have recognized the Helicon abbreviations. The details of the filters and excitation source would be very interesting to know. Maybe you have referred to it in another post somewhere that you could refer us to? |
Excitation Filter: 352 – 402 nm single band bandpass filter
Dichroic Mirror: 414nm
Emission Filter: 437nm long pass edge filter |
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Lou Jost
Joined: 04 Sep 2015
Posts: 2859
Location: Ecuador
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Pau
Site Admin
Joined: 20 Jan 2010
Posts: 4528
Location: Valencia, Spain
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| Posted: Thu Apr 12, 2018 12:11 am Post subject: |
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Excellent images, Waldo
This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching?
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Pau |
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micro_pix
Joined: 11 May 2012
Posts: 141
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| Posted: Thu Apr 12, 2018 3:07 am Post subject: |
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Stunning images.
David |
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pwnell
Joined: 18 Dec 2009
Posts: 1987
Location: Tsawwassen, Canada
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| Posted: Thu Apr 12, 2018 8:48 am Post subject: |
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| Pau wrote: | Excellent images, Waldo
This is one of my favorite subjects but I've been unable to get results like yours because the fast photobleaching of the chlorophyll, both with UV and blue excitation.
Please, could you tell me the ISO setting and exposure time of the pictures?. Maybe my excitation intensity is too high although I doubt that my LEDs will provide more intensity than your HBO.
Did you use ND filters or any other trick to avoid photobleaching? |
Thanks Pau. I agree - photobleaching is definitely a concern here. My ISO was 400. However I had a 1.6 second exposure time per frame. My X-Cite 120 was set to its lowest level like all my photos. I definitely do engage ND filters - but I never record which ones. I have a 0.25 and 0.6. Sometimes I'll use both, but this image lets me believe I only engaged the 0.6 or the 0.25. |
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Pau
Site Admin
Joined: 20 Jan 2010
Posts: 4528
Location: Valencia, Spain
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| Posted: Thu Apr 12, 2018 9:03 am Post subject: |
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Thank you very much, this is relevant info, I usually shot at ISO 100 or 200 and get exposures about 1s, so I'll need to try lowering the excitation by means of ND
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Pau |
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Roel Wijtmans
Joined: 23 Nov 2016
Posts: 55
Location: Sweden
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| Posted: Thu Apr 12, 2018 9:14 am Post subject: |
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| Stunning photos! |
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Sumguy01
Joined: 28 Jan 2013
Posts: 1187
Location: Ketchikan Alaska USA
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| Posted: Sun Apr 15, 2018 10:42 am Post subject: |
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 Amazing set.
Thanks for sharing. |
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