www.photomacrography.net :: View topic - Aphid Identification
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Aphid Identification

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
glmory



Joined: 30 Dec 2016
Posts: 25

PostPosted: Tue Mar 06, 2018 10:25 pm    Post subject: Aphid Identification Reply with quote

I have been working quite a bit of macro and micro photography into my iNaturalist observations this past year.

When I started photographing aphids, the experts I talked to didn't think more obscure species could be identified from photos. As I learned more, I saw their point. However if you work through a key, photographing every feature in the key it is usually possible to do. You just need photos of the right features with the right measurements.

Here is an aphid I have yet to get to species conclusively(https://www.inaturalist.org/observations/10072629). It is either Chaitophorus utahensis or a similar but undescribed species in that genus.

I start with macro photos of the aphids:



Then I slide mount them and take an overall photo using an Amscope 4x objective. On that I take a bunch of measurements which I know are commonly required to work through keys:



Then I go through a key taking photos of other characteristics which are needed to make it to an identification. When I need good quality I use a Nikon E Plan PH3 40x objective





It is on a Labophot microscope with brightfield dark field and phase contrast. I was too cheap for a trinocular head so I just put a dovetail adapter and a bunch of extension tubes on it.


[/img]

All the photos are taken with an a6300 camera. I put it in silent shutter mode on the camera app, then have continuously take photos while I move the focus.

I stack in Zerene Stacker then add scale bars and clean up the background in Photoshop.
Back to top
View user's profile Send private message
clengman



Joined: 10 Feb 2018
Posts: 2

PostPosted: Wed Mar 07, 2018 10:36 am    Post subject: Reply with quote

Very nice! What method do you use for preparing your aphids for TL? Are you making permanent mounts?
Back to top
View user's profile Send private message
glmory



Joined: 30 Dec 2016
Posts: 25

PostPosted: Wed Mar 07, 2018 12:59 pm    Post subject: Canada balsam Reply with quote

Yeah, they are permanently mounted. I learned to make mounts in Canada Balsam by watching some online videos.

Here is a description of the procedure:
https://www.inaturalist.org/journal/glmory/11320-aphid-identification-ii
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group