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Jumping Spider and fluorescence

 
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WalterD



Joined: 06 Jul 2015
Posts: 354
Location: Rotterdam, the Netherlands

PostPosted: Fri Mar 09, 2018 2:12 am    Post subject: Jumping Spider and fluorescence Reply with quote

Jumping Spiders are kind of cute compared to other spiders. This one (less than 2mm small) was found on my desk, it might have been inhabitant of the lichen branch from the previous post.
It's always -or let's say most of the time- a surprise whether and how a new object will respond to fluorescent light. In this case the image remained dark, except the pedipalps that illuminated the rest of it.
(a reflection of both can be found in the eyes). I combined this with diffused green led light, to improve the appearance of the non-fluorescent items.
For the rest the blue filterblock with the YOC6-3 exciter were used plus 2 less important ones. Objective F 23,2/Na 0,17(which is 7x). Canon EOS 5D Mk ii. Stacked with Zerene (dmap substacks).

To give you a better idea of this phenomenon I added an unstacked ventral view image showing the powerful radiation. Many details were lost due to compression, check out http://waltermachielsen.com/jumping-spider-fluorescence/




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Last edited by WalterD on Sat Mar 24, 2018 11:35 am; edited 6 times in total
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Robert Berdan



Joined: 18 Oct 2017
Posts: 25
Location: Calgary

PostPosted: Fri Mar 09, 2018 9:07 pm    Post subject: Nice lighting Reply with quote

Very nice lighting and very different - nice photos.
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GaryB



Joined: 29 Jul 2016
Posts: 519

PostPosted: Sun Mar 11, 2018 2:03 am    Post subject: Reply with quote

They look great!
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WalterD



Joined: 06 Jul 2015
Posts: 354
Location: Rotterdam, the Netherlands

PostPosted: Sun Mar 11, 2018 6:51 am    Post subject: Reply with quote

Glad you like them!
Three days later with the same setup the eyes were responding as well. This resulted in a more bizarre image. Not sure what's the explanation, however the previous picture was made when the spider was just dead. After three days without using any fixative, decay can be expected. The "hydraulic pressure" that kept the spider in shape is gone and the eyes seem to have lost some of their their ball shape too. However, in this picture it's easy to spot the additional set of eyes they've got in the side of their heads. The other (smaller) ball shaped item next to that cannot be an eye though.



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billben74



Joined: 09 Apr 2016
Posts: 145
Location: Cambridge, UK

PostPosted: Sun Mar 11, 2018 10:08 am    Post subject: Reply with quote

Really interesting image and post.
Many thanks.

I find that the eyes of spider and insects do often become white as they dry out. I always do the eyes first when I doing a stack/stitch job to catch them nice and black.
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WalterD



Joined: 06 Jul 2015
Posts: 354
Location: Rotterdam, the Netherlands

PostPosted: Sun Mar 11, 2018 11:44 am    Post subject: Reply with quote

Hi Billben74,

Thanks for your reply. Indeed this may be the combination of dried eyes and fluorescence. Meantime I've edited the 1st image as the colour green was a bit too dominant, all in all slightly on the dark side as well. What do you think?

Regards,
Walter
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GaryB



Joined: 29 Jul 2016
Posts: 519

PostPosted: Sun Mar 11, 2018 4:00 pm    Post subject: Reply with quote

Ok, now we're into walking dead territory.. Shocked
Laughing
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Jacek



Joined: 02 Oct 2011
Posts: 4962
Location: Poland

PostPosted: Mon Mar 12, 2018 3:22 am    Post subject: Reply with quote

Very nice
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WalterD



Joined: 06 Jul 2015
Posts: 354
Location: Rotterdam, the Netherlands

PostPosted: Mon Mar 12, 2018 10:32 pm    Post subject: Reply with quote

Quote:
Ok, now we're into walking dead territory..

Hahaha! Very Happy

Thanks Jacek!

Regards,

Walter
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 4920
Location: Uruguay - Montevideo - America del Sur

PostPosted: Thu Mar 15, 2018 5:56 am    Post subject: Reply with quote

SUPER!
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sushidelic



Joined: 10 Jun 2016
Posts: 192

PostPosted: Fri Mar 16, 2018 2:13 pm    Post subject: Reply with quote

Ok, now this one does look scary.

Great work!
Michael
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vasselle



Joined: 05 Jan 2014
Posts: 1425
Location: France

PostPosted: Fri Mar 16, 2018 3:27 pm    Post subject: Reply with quote

Bonjour
Superbes images de ces jolis yeux
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WalterD



Joined: 06 Jul 2015
Posts: 354
Location: Rotterdam, the Netherlands

PostPosted: Sat Mar 17, 2018 5:41 am    Post subject: Reply with quote

Firstly many thanks for your pleasant feedback.
Unfortunately the spider did not really return; it could not be brought back to life.
However these pictures are taken from the same spider. The first image (no.4) with the Orthoplan Ultropak (Leitz) setup. Half of the third pair of eyes in the side of the head can be noticed. In the eyes you can see the typical reflection of the Ultropak -darkfield, reflected light- objective. Basically every eye in any jumping spider picture exactly mirrors the actual setup. (diffusor, microscope/camera objective lens, illumination). As mentioned, in the fluorescent images the fluorescence induced radiation from the palps is the actual light source and as such reflected in the eyes. Jumping back to image no.4 I've tried to indicate how small the spider was for real. The object is sitting here on a flat toothpick. The picture was taken after the "zombie" picture, besides the curled up legs I did not notice much decay here.







Image no.5 -a bit more dramatic- was actually the first set taken. In this picture the focus is more on the palps (avoided white clipping here) and less on the eyes. The vague hairs were taken from one single other picture, together with the stack merged to this final image.



Image no.6 is a stack of what has been shown in no.2. It is showing a ventral view of a part of the spider. Again the palps illuminate the image, furthermore noted knife-like mouth parts, similar to some insects.



Still I've got a couple of questions, maybe one of you has got an answer:
-Does anybody know this phenomenon of autofluorescent palps on spiders?
-Do "dwarf" jumping spiders exist? This one got scales instead of hairs, was wondering whether it is just very young or the scales will remain ?
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