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Paramecium caudatum

 
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sushidelic



Joined: 10 Jun 2016
Posts: 176

PostPosted: Tue Apr 10, 2018 7:42 am    Post subject: Paramecium caudatum Reply with quote

Olympus UPLANAPO 40x, U-DICT, 1/2000sec, no flash...





Best regards,
Michael
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vasselle



Joined: 05 Jan 2014
Posts: 1398
Location: France

PostPosted: Tue Apr 10, 2018 3:36 pm    Post subject: Reply with quote

Magnifique
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micro_pix



Joined: 11 May 2012
Posts: 136

PostPosted: Tue Apr 10, 2018 4:52 pm    Post subject: Reply with quote

Wonderful photos!

How do you get 1/2000 sec with no flash in DIC with a 40X objective? It is very impressive.
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razashaikh



Joined: 14 Nov 2017
Posts: 103
Location: India

PostPosted: Tue Apr 10, 2018 9:44 pm    Post subject: Reply with quote

Very nice observation.
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Jacek



Joined: 02 Oct 2011
Posts: 4913
Location: Poland

PostPosted: Wed Apr 11, 2018 4:33 am    Post subject: Reply with quote

Very nice
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Cactusdave



Joined: 09 Jun 2009
Posts: 1606
Location: Bromley, Kent, UK

PostPosted: Thu Apr 12, 2018 12:20 am    Post subject: Reply with quote

Beautiful, I'd like to repeat micro_pix's question

Quote:
How do you get 1/2000 sec with no flash in DIC with a 40X objective? It is very impressive.


Only I was thinking "How the expletive do you get ..... etc". Shocked Very Happy
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sushidelic



Joined: 10 Jun 2016
Posts: 176

PostPosted: Thu Apr 12, 2018 1:32 am    Post subject: Reply with quote

Hi and thanks for your answers!

As I do not have a flash adaption yet (biiiig thing on the wish list), I am forced to use the continuous light of the scope.
Here it was a BX50 with a U-LH100 lamp house, so 100W halogen.

For pond peeping pictures, I remove all filters (ND, LBD...) from the light path and set the illumination power to max. Color temperature is then compensated by white balancing the camera. The only "light loss" is the trinocular, as I still direct 20% of the light to the eyepieces and 80% to the cam.

I use a Nikon D800 for taking the pictures, on a U-SWTR-2 tube and project directly to the sensor, no projective used. Certainly this results in a vignetted image on a FF sensor, but more light is directed towards one pixel due to the smaller image spot.

DIC is set in a way that still a lot of light passes the filters (so not too close to "DF / crossed nicols", but DIC relief is still satisfying.

With all the above I am able to use continuous light, 40x and DIC with ISO ~1200/1600 and exposure times of 1/1000 - 1/2000 seconds.
This is needed in two regards - freezing the specimen, cilia etc. AND to prevent the earthquake like mirror shock of the D800 when used without exposure delay.

Hope this helps and best regards,
Michael
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micro_pix



Joined: 11 May 2012
Posts: 136

PostPosted: Thu Apr 12, 2018 3:23 am    Post subject: Reply with quote

Thanks for the explanation. I use a BH2 BHS so I do have the 100w halogen but I do use the projection eyepiece. I must admit I haven’t used the high asa settings as I thought there would be too much noise but I’ll give that a try.

David
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Cactusdave



Joined: 09 Jun 2009
Posts: 1606
Location: Bromley, Kent, UK

PostPosted: Fri Apr 13, 2018 7:09 am    Post subject: Reply with quote

My thanks too Michael. You are lucky to have the combination of a really powerful light source and a camera with an excellent high ISO performance. Great work, and proves it is possible to get this kind of image without flash.
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