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Rhizocarpon lecanorinum

 
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ralfwagner



Joined: 05 Aug 2006
Posts: 422
Location: Germany, Duesseldorf

PostPosted: Sat Feb 10, 2018 12:28 pm    Post subject: Rhizocarpon lecanorinum Reply with quote

Hello,

this crustose lichen is from an old lead pit. It is growing on silicate rocks.

- Closeup thallus, 105 mm makroobjective with 36 mm extension ring, 45 picture stack with Helicon



- section apothecium, LCB (lactophenol-cottonblue) stain





- Spore, 43 x 16 ┬Ám



- Orange fluorescence of the rhizocarpic acid that is situated in the cortex. Beneath the red chlorophyll autofluorescence of the photobiont, a green algae.


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Ralf

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Sumguy01



Joined: 28 Jan 2013
Posts: 1085
Location: Ketchikan Alaska USA

PostPosted: Sat Feb 10, 2018 2:21 pm    Post subject: Reply with quote

Very Happy Very interesting.
Thanks for sharing.
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micro_pix



Joined: 11 May 2012
Posts: 141

PostPosted: Sun Feb 11, 2018 4:14 am    Post subject: Reply with quote

Very interesting, I particularly like the flourescence image.
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Mike



Joined: 04 Aug 2006
Posts: 86
Location: Northeast Ohio

PostPosted: Sun Feb 11, 2018 7:13 am    Post subject: Reply with quote

Hello Ralf,

Interesting and educational series - appreciate how you go from macro to micro with photos/info. Might have to look at the various lichens around my place.

Thanks,
Mike
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Jacek



Joined: 02 Oct 2011
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Location: Poland

PostPosted: Mon Feb 12, 2018 12:23 am    Post subject: Reply with quote

Very nice
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ralfwagner



Joined: 05 Aug 2006
Posts: 422
Location: Germany, Duesseldorf

PostPosted: Tue Feb 13, 2018 3:03 am    Post subject: Reply with quote

Mike wrote:
Might have to look at the various lichens around my place.


Mike, this is a very good iadea and will bring you much to see and learn about nature.

Thanks @ all for your interest in lichens.
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Ralf

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Pau
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Joined: 20 Jan 2010
Posts: 4317
Location: Valencia, Spain

PostPosted: Tue Feb 13, 2018 4:33 am    Post subject: Reply with quote

Excellent work!
Quote:
- Orange fluorescence of the rhizocarpic acid that is situated in the cortex. Beneath the red chlorophyll autofluorescence of the photobiont, a green algae.

Is this rizocarpic acid auto fluorescent? Is it present in many lichen species? What excitation wavelength did you use?
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Pitufo



Joined: 21 Jun 2015
Posts: 193
Location: United Kingdom

PostPosted: Tue Feb 13, 2018 5:27 am    Post subject: Reply with quote

It seems that lichens produce quite a lot of different colours with UV autofluorescence. See here.

https://canadianmuseumofnature.wordpress.com/2014/08/28/fluorescent-lichens-dazzling-creatures-of-light/
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ChrisR
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Joined: 14 Mar 2009
Posts: 7689
Location: Near London, UK

PostPosted: Tue Feb 13, 2018 5:50 am    Post subject: Reply with quote

I let my schoolkids discover that, with a 365mm nichia-powered torch. It was declared to be "well cool". Very Happy
I didn't have anything as psychedelic as that article shows, though.
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ralfwagner



Joined: 05 Aug 2006
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Location: Germany, Duesseldorf

PostPosted: Tue Feb 13, 2018 6:42 am    Post subject: Reply with quote

Pau wrote:

Is this rizocarpic acid auto fluorescent? Is it present in many lichen species? What excitation wavelength did you use?


Rhizocarpic acid is auto fluorescent. I used a "royal blue led" @ 440 nm for exitation. Look here for the chemical structure: http://www.chemicalbook.com/ChemicalProductProperty_EN_CB4202378.htm

Rhizocarpic acid is present in all yellow or yellow-green species of the genus Rhizocarpon. You will find rhizocarpic acid also in many Acarospora species and in some Lecanora spec. (e.g. Lecanora epanora) and in Lecidea lucida. This list is not complete but just what is mentioned in my literature.

As mentioned by Pitufo, there are many auto fluorescent lichen substances. For example many Cladonia spec. show a white fluorescence caused by sqamatic acid, Xanthoria parietina shows the orange fluorescence of parietin.
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Pau
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Joined: 20 Jan 2010
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PostPosted: Tue Feb 13, 2018 9:08 am    Post subject: Reply with quote

Thanks for the info, Ralf
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