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Some Diatoms Mounted in Realgar
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Cactusdave



Joined: 09 Jun 2009
Posts: 1619
Location: Bromley, Kent, UK

PostPosted: Sat Jan 27, 2018 7:51 am    Post subject: Reply with quote

The designation on the Leitz NPL Fluotar X50, 1.0, oil of 160/- means that it is not specifically corrected for a 0.17mm thick coverslip. The Zeiss Planapo X40, 1.0, oil, iris is similarly designated 160/-. This does not mean they cannot be used with coverslips of 0.17mm, it simply means that the lens is not specifically corrected for this thickness of glass. There might be sound marketing reasons for this. These lenses would be good for examining fixed, but not coversliped blood films in a Path. lab. This was potentially a big market.

I have successfully used both the Leitz lens and the Zeiss to look at modern high quality diatom mounts where the coverslip thickness is likely to be of the order of 0.17+/- 0.02mm with no problems of working distance. With coverslips significantly thicker than this, any oil immersion lens (and some dry Planapos,) may be unable to reach focus. I can only reiterate the advice I was given a long time ago. When working with immersion lenses, or any lenses with tiny working distance, exercise great care when finding the focal plane on any slide where you can't guarantee the thickness of the coverslip. There may not be one before you hit the coverslip! Crying or Very sad
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Pau
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Joined: 20 Jan 2010
Posts: 4279
Location: Valencia, Spain

PostPosted: Sat Jan 27, 2018 9:52 am    Post subject: Reply with quote

I subscribe almost all Cactuscave says.

Quote:
Personally I would never directly revolve an oil immersion objective into place. I wouldn't trust parfocality to that extent, and I don't think it's good practice.

Formerly I proceed like you and I still do in some cases but I find easier and also better to avoid air bubbles to directly revolver the objective because I have very good parfocality between Leitz objectives. This doesn't apply when I mix Zeiss and Leitz objectives like I sometimes do with the NPL Fluotar 50/1.00 and Planapo 63/1.4
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