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Custom phase contast diatom
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maikl36911



Joined: 18 Apr 2014
Posts: 65
Location: Russia,Moskow

PostPosted: Sat Jan 06, 2018 2:07 pm    Post subject: Custom phase contast diatom Reply with quote



lomo 40 0.65

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Sumguy01



Joined: 28 Jan 2013
Posts: 1159
Location: Ketchikan Alaska USA

PostPosted: Sat Jan 06, 2018 6:30 pm    Post subject: Reply with quote

Very Happy Very nice.
Thanks for sharing
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nanometer



Joined: 30 Apr 2016
Posts: 153
Location: Tucson, AZ

PostPosted: Sun Jan 07, 2018 9:23 pm    Post subject: Reply with quote

Stunning both in color and detail.
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maikl36911



Joined: 18 Apr 2014
Posts: 65
Location: Russia,Moskow

PostPosted: Mon Jan 08, 2018 5:13 am    Post subject: Reply with quote

nanometer wrote:
Stunning both in color and detail.


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JH



Joined: 09 Mar 2013
Posts: 1171
Location: Vallentuna, Stockholm, Sweden

PostPosted: Mon Jan 08, 2018 9:26 am    Post subject: Reply with quote

Nice and clear.
What is custom phase contrast?

Best regards
Jörgen Hellberg
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5011
Location: Uruguay - Montevideo - America del Sur

PostPosted: Tue Jan 09, 2018 9:46 am    Post subject: Reply with quote

Nice!!!!!
Are you using polarized light? why they have colors?
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Cactusdave



Joined: 09 Jun 2009
Posts: 1631
Location: Bromley, Kent, UK

PostPosted: Tue Jan 09, 2018 12:02 pm    Post subject: Reply with quote

Very interesting and nice images. I would also like to know how this 'magic' is worked. It reminds me of the near mythical Leitz 'colour Heine' phase contrast. Shocked
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Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear
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maikl36911



Joined: 18 Apr 2014
Posts: 65
Location: Russia,Moskow

PostPosted: Tue Jan 09, 2018 1:20 pm    Post subject: Reply with quote

carlos.uruguay wrote:
Nice!!!!!
Are you using polarized light? why they have colors?


only phase contast,no polarization

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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5011
Location: Uruguay - Montevideo - America del Sur

PostPosted: Tue Jan 09, 2018 1:56 pm    Post subject: Reply with quote

and the colors where they come from? d'oh!
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maikl36911



Joined: 18 Apr 2014
Posts: 65
Location: Russia,Moskow

PostPosted: Tue Jan 09, 2018 2:12 pm    Post subject: Reply with quote

carlos.uruguay wrote:
and the colors where they come from? d'oh!


https://en.wikipedia.org/wiki/Phase-contrast_microscopy
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5011
Location: Uruguay - Montevideo - America del Sur

PostPosted: Tue Jan 09, 2018 2:38 pm    Post subject: Reply with quote

I still do not understand the colors, sorry, thanks anyway!
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Cactusdave



Joined: 09 Jun 2009
Posts: 1631
Location: Bromley, Kent, UK

PostPosted: Wed Jan 10, 2018 1:44 am    Post subject: Reply with quote

I don't blame the poster if he doesn't want to reveal his exact methods, especially as he has something that is working in a very nice and rather unique way. Colour phase contrast has certainly been described in the past. I'm aware of Leitz Heine, Reichert, and Zernicke versions and I believe Nikon attempted something similar with their Interference Phase Contrast Unit. There is some information and discussion here:

http://bettermicroscopy.blogspot.co.uk/2017/09/heine-ipc-new-color-phase-contrast.html

Maikl's images are as good or better than any I have seen published on the web for colour phase contrast, so yes I would still love to know how it's done. Very Happy
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maikl36911



Joined: 18 Apr 2014
Posts: 65
Location: Russia,Moskow

PostPosted: Wed Jan 10, 2018 3:54 am    Post subject: Reply with quote

Cactusdave wrote:
I don't blame the poster if he doesn't want to reveal his exact methods, especially as he has something that is working in a very nice and rather unique way. Colour phase contrast has certainly been described in the past. I'm aware of Leitz Heine, Reichert, and Zernicke versions and I believe Nikon attempted something similar with their Interference Phase Contrast Unit. There is some information and discussion here:

http://bettermicroscopy.blogspot.co.uk/2017/09/heine-ipc-new-color-phase-contrast.html

Maikl's images are as good or better than any I have seen published on the web for colour phase contrast, so yes I would still love to know how it's done. Very Happy


thanks

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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5011
Location: Uruguay - Montevideo - America del Sur

PostPosted: Wed Jan 10, 2018 4:44 am    Post subject: Reply with quote

Why should not the methods be shared ???
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JH



Joined: 09 Mar 2013
Posts: 1171
Location: Vallentuna, Stockholm, Sweden

PostPosted: Wed Jan 10, 2018 1:09 pm    Post subject: Reply with quote

Thanks for sharing this beautiful pictures!
Best regards
Jörgen Hellberg
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