I have been playing with my fluorescence set up recently. To date I have built a simple 1W blue LED lightsource and I have made my 445nm laser set up safer. I have a new laser module on a heatsink with a low power lens so the beam diverges nicely when I plug it into the lamp holder on my Olympus microscope. I use dark ground illumination and a 450nm or 500nm blocking filter. The advantage of using a laser is that it is monochromatic so even the 450nm blocking filter gets rid of almost all of the stray blue light. Anyway here are a couple of the preliminary images.
1. is an old prep of daffodil pollen (unstained).
2 & 3 are bacteria stained with acridine orange.
The images were photographed using an EOS 6D at 1600 ISO and exposures of 4 to 8 seconds.
An improved fluorescence set up
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- carlos.uruguay
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