Diatom Threw Slide Mix

sushidelic · Post by **sushidelic** » Mon Mar 27, 2017 2:19 am

Hello,
her are some pics from a recent threw slide set.
All credits for the slides go to Anne.
All images were taken with the Olympus SPlan Apo 60x/1.4, green filtration, NFK 3.3x and Nikon D800, processing in RawTherapee, stacked in Zerene and polished in Photoshop.

1:
Pinnularia neomajor, ~170µm

Areolae, crop from the image above, pore distance between 170 - 190nm

Rhopalodia spec., ~180µm

Crop from the image above

A mandala

And a really small one

Thanks for looking,
Michael

GaryB · Post by **GaryB** » Mon Mar 27, 2017 3:37 am

Very clean set, nicely lit.

zzffnn · Post by **zzffnn** » Mon Mar 27, 2017 4:49 am

Very nice, thank you for sharing, Michael!

Were they imaged in plain transmitted brightfield? Did you use green filter to reduce flare and enhance contrast?

sushidelic · Post by **sushidelic** » Mon Mar 27, 2017 6:21 am

Thanks for your answers!

It's not really "plain" transmitted light. A little bit oblique, and I was playing with the crossed polarizers to achieve maximum detail/contrast. If you don't mind ending up with monochrome images, green light is better in terms of flaring and resolution than just using the green channel of a "white" light image. Due to interference effects, other wavelengths also tend to creep into the green channel, so the result with white light is usually a bit inferior, depends on the circumstances. For max resolution, it would be best to use 365 nm UV light and a modded DSLR and according filtration.

Best regards,
Michael

carlos.uruguay · Post by **carlos.uruguay** » Mon Mar 27, 2017 7:12 am

SUPER!

Olympusman · Post by **Olympusman** » Mon Mar 27, 2017 11:28 am

Very very nice.

Mike

Jacek · Post by **Jacek** » Mon Mar 27, 2017 12:01 pm

Very nice, good job

curt0909 · Post by **curt0909** » Mon Mar 27, 2017 12:38 pm

Some of the best diatom images I've seen. Superb!

JH · Post by JH » Mon Mar 27, 2017 1:26 pm

Nice!

Regards Jörgen

Cactusdave · Post by **Cactusdave** » Mon Mar 27, 2017 1:31 pm

Very good indeed. I honestly don't think you can do much better than that with a light microscope.

sushidelic · Post by **sushidelic** » Mon Mar 27, 2017 2:12 pm

Thanks a lot for all your comments!

And well, it's nearly midnight over here in Germany, so I hope it's ok if I break the six-image-a-day-limit by a single one...

Any similarities to well known symbols are purely coincidental.

Best regards,
Michael

Smokedaddy · Post by **Smokedaddy** » Mon Mar 27, 2017 8:12 pm

I love the first image. It totally stands out, excellent lighting effects, very well done.

-JW:

Sumguy01 · Post by **Sumguy01** » Mon Mar 27, 2017 8:55 pm

Nice set.
#1 is excellent.
Thanks for sharing.

sushidelic · Post by **sushidelic** » Tue Mar 28, 2017 11:09 am

Thanks again for your comments,
here are another two:

Cymatopleura elliptica var hibernica (W. Smith) Van Heurck 1896

Diploneis alpina Meister

Thanks for looking!

Best regards,
Michael

Edit: Thanks to the help of the diatom-experts on the german microscopy-forum, i was able to add the identification

GaryB · Post by **GaryB** » Tue Mar 28, 2017 2:04 pm

Beautiful!

Diatom Threw Slide Mix

Diatom Threw Slide Mix

Diatom strew